Shopping Cart » PARP in vivo PDA   » 3D Assays   » Stem Cell Assays   » Cell Invasion   » PathClear® BME   » PARP   » CometAssay®  Home » Angiogenesis & Cell Differentiation Angiogenesis & Cell Differentiation Cultrex® 3D Culture MatrixTM Cell Harvesting Kit 3D Cultures exhibit cellular behaviors and morphologies similar to those seen in vivo, however, the adaptation of these models for studying biochemical processes has been impeded by the challenge of separating intact cells from extracellular proteins comprising the hydrogel. Commonly, proteases are employed to degrade these extracellular proteins, however, proteases also degrade proteins on the cell surface and protease activity may carry over into lysate preparations. Non-enzymatic methodologies have also been described for depolymerizing extracellular matrix proteins, although the implementation of these protocols remains problematic for some researchers. Trevigen's Cultrex® 3D Culture Cell Harvesting Kit provides an optimized and standardized solution for the isolation and normalization of cell lysates from 3D Culture MatrixTM BME or Laminin I for subsequent biochemical analysis.   Applications: Isolating cells from 3-D culture for biochemical analysis.   Components: Part # Component Size   3445-005-01 3D Culture Matrix 5 ml   3448-020-01 10X Cell Harvesting Buffer 100 ml   3448-020-02 10X Cell Wash Buffer 100 ml   3448-020-03 Sample Buffer 10 ml   3448-020-04 10X Loading Buffer 1 ml   Storage: Components are stored at room temperature and -20°C   Top   Ordering Information Catalog #: Price: 3448-020-K Cultrex 3D Culture Cell Harvesting Kit (human/mouse), 20 Tests $126.00 Trevigen Products are now available for ordering through - AND - Download Protocols Related Products Cultrex 3D Culture 96 Well Collagen I Cell Proliferation Assay Cultrex 3D Culture 96 Well BME Cell Proliferation Assay Cultrex 3D Culture Cell Proliferation Assay Core Kit Cultrex 3D Culture 96 Well Laminin I Cell Proliferation Assay Cultrex 3D Culture Matrix Rat Collagen I Cultrex 3D Culture Matrix Laminin I Cultrex 3D Culture Matrix BME TREVIGEN INC. :: 8405 HELGERMAN CT. :: GAITHERSBURG, MD 20877 1.800.TREVIGEN :: 1.800.873.8443 :: (FAX) 1.301.560.4973 :: INFO@TREVIGEN.COM :: SITE MAP