Shopping Cart » Quantitative PAR ELISA   » 3D Assays   » Stem Cell Assays   » Cell Invasion   » PathClear® BME   » PARP   » CometAssay®  Home » Angiogenesis & Cell Differentiation Angiogenesis & Cell Differentiation CultreCoat® 96 well BME Cell Invasion Assays Trevigen's CultreCoat® 96 Well Cell Invasion Assays were created in an effort to accelerate in vitro screening for modulators of these processes, and to further enable the investigation of pathways that influence cell invasion through extracellular matrices. These assays offer three distinct standardized, high throughput formats for quantitating the degree to which invasive cells penetrate a barrier, consisting of basement membrane components in response to chemoattractants, and/or inhibiting com-pounds. Trevigen has developed an in vitro cell invasion assay system composed of three different coating densities that may be used for cell models with either high, medium, or low invasive potentials. If the optimal coating density is not known in advance, the requirements for each cell model should be determined by using the CultreCoat® 96 Well BME Optimization Cell Invasion Assay. The CultreCoat® 96 Well BME Cell Invasion Optimization Assay (Catalog #: 3484-096-K) contains three different coating concentrations and uncoated wells on one plate to compare invasion to basal migration and determine the optimal coating density for your cell model. Optimal invasion should be 60-80% of basal migration, demonstrating that the BME is providing a barrier effect and giving the maximum signal to noise ratio and dynamic range. Once assay conditions are established, the corresponding 96 well invasion assay with the optimal coating density may be used for screening. The low density BME coating is for cell models that exhibit a nominal invasive capacity, the medium density BME coating is for cell models that exhibit an intermediate invasive capacity and the high density BME coating is for cell models that exhibit an accelerated invasive capacity. Applications: Cell Migration/Invasion Assays   Components: Part # Component Size   *1 3484-096-01 96 Well BME-Coated Optimization Cell Invasion Chamber each   *2 3481-096-01 96 Well Low BME Cell Invasion Chamber each   *3 3482-096-01 96 Well Medium BME Cell Invasion Chamber each   *4 3483-096-01 96 Well High BME Cell Invasion Chamber each   3455-096-04 25X Cell Wash Buffer 2 x 1.5ml   3455-096-04 10X Cell Dissociation Solution 2 x 1.5ml   4892-010-01 Calcein-AM 50 µg   *1 Included with Catalog#: 3484-096-K *2 Included with Catalog#: 3481-096-K *3 Included with Catalog#: 3482-096-K *4 Included with Catalog#: 3483-096-K   Storage Components are stored at -20°C and 4°C.   References: 1. Tamilarasan KP, Kolluru GK, Rajaram M, Indhumathy M, Saranya R, Chatterjee S. 2006. Thalidomide attenuates nitric oxide mediated angiogenesis by blocking migration of endothelial cells. BMC Cell Biol. 7:17. 2. Borghesani PR, Peyrin JM, Klein R, Rubin J, Carter AR, Schwartz PM, Luster A, Corfas G, Segal RA. 2002. BDNF stimulates migration of cerebellar granule cells. Development 129:1435-1442. 3. Mohan K, Ding Z, Hanly J, Issekutz TB. 2002 IFN-gamma-inducible T cell alpha chemoattractant is a potent stimulator of normal human blood T lymphocyte transendothelial migration: differential regulation by IFN-gamma and TNF-alpha. J Immunol. 168:6420-6428. Top   Ordering Information Catalog #: Price: 3484-096-K CultreCoatŪ 96 Well BME-Coated Cell Invasion Optimization Assay, 96 Samples $475.00 3481-096-K CultreCoatŪ 96 well Low BME Cell Invasion Assay, 96 Samples $475.00 3482-096-K CultreCoatŪ 96 Well Medium BME Cell Invasion Assay, 96 Samples $475.00 3483-096-K CultreCoatŪ 96 Well High BME Cell Invasion Assay, 96 Samples $475.00 Trevigen Products are now available for ordering through - AND - Download Protocol Select Protocol 3481-096-K 3482-096-K 3483-096-K 3484-096-K Related Products CultreCoatŪ 24 Well BME-Coated Cell Invasion Assay CultreCoatŪ Vitronectin 96 Well Cell Adhesion Assay CultreCoatŪ Collagen IV 96 Well Cell Adhesion Assay CultreCoatŪ Laminin I 96 Well Cell Adhesion Assay CultreCoatŪ Adhesion Protein Array Kit CultreCoatŪ Fibronectin 96 Well Cell Adhesion Assay CultreCoatŪ Collagen I 96 Well Cell Adhesion Assay CultreCoatŪ BME 96 Well Adhesion Assay TREVIGEN INC. :: 8405 HELGERMAN CT. :: GAITHERSBURG, MD 20877 1.800.TREVIGEN :: 1.800.873.8443 :: (FAX) 1.301.560.4973 :: INFO@TREVIGEN.COM :: SITE MAP