Shopping Cart » DIVAATM     » Cell Invasion     » Cell Migration     » PARP     » CometAssayTM     » HT 8-oxo-dG ELISA  Home » Angiogenesis & Cell Differentiation Angiogenesis & Cell Differentiation Cultrex® 24 Well Cell Migration Assay The Cultrex® Cell Migration Assay was created in an effort to accelerate the screening process for compounds that influence chemotaxis, which is a fundamental function of cellular processes such as angiogenesis, embryonic development, immune response, and wound healing. This assay offers a flexible, standardized, high throughput format for quantitating the degree to which cells migrate in vitro in response to chemoattractants and/or inhibiting compounds. Trevigen’s Cultrex® 24 well Cell Migration Assay utilizes a simplified Boyden chamber design with an 8 mm polyethylene terephthalate (PET) membrane. Ports within the migration chamber (top) allow access to the assay chamber (bottom). The inserts are sufficiently large to allow for subsequent analysis of migrating subpopulations, in response to chemokines, cytokines, drugs or other compounds of interest. Detection of cell invasion is quantified using Calcein-AM. Calcein-AM is internalized, and intracellular esterases cleave the acetomethylester (AM) moiety to generate free Calcein. Free Calcein fluoresces brightly, and this fluorescence may be used to quantitate the number of cells that have migrated using a standard curve. Applications: To quantitate cell migration   Components: Part # Component Size   3455-024-01 24 well cell invasion/migration plates 2 Plates   3455-096-04 25X Cell Wash Buffer 2 x 1.5 ml   3455-096-05 10X Cell Dissociation Solution 2 x 1.5 ml   4892-010-01 Calcein AM 50 µg   Storage: Components are stored at -20ºC, 4ºC, and at room temperature.   References: 1. Tamilarasan KP, Kolluru GK, Rajaram M, Indhumathy M, Saranya R, Chatterjee S. 2006. Thalidomide attenuates nitric oxide mediated angiogenesis by blocking migration of endothelial cells. BMC Cell Biol. 7:17. 2. Borghesani PR, Peyrin JM, Klein R, Rubin J, Carter AR, Schwartz PM, Luster A, Corfas G, Segal RA. 2002. BDNF stimulates migration of cerebellar granule cells. Development 129:1435-1442. 3. Mohan K, Ding Z, Hanly J, Issekutz TB. 2002 IFN-gamma-inducible T cell alpha chemoattractant is a potent stimulator of normal human blood T lymphocyte transendothelial migration: differential regulation by IFN-gamma and TNF-alpha. J Immunol. 168:6420-6428. Top   Ordering Information Catalog #: Price: 3465-024-K Cultrex 24 Well Cell Migration Assay, 24 Samples $295.00 Trevigen Products are now available for ordering through Download Protocol Related Products Cultrex 24 Well BME Cell Invasion Assay Cultrex 24 Well Laminin I Cell Invasion Assay Cultrex 24 Well Collagen I Cell Invasion Assay Cultrex 24 Well Collagen IV Cell Invasion Assay Cultrex 96 Well Cell Migration Assay Cultrex 96 Well BME Cell Invasion Assay Cultrex 96 Well Laminin I Cell Invasion Assay Cultrex 96 Well Collagen I Cell Invasion Assay Cultrex 96 Well Collagen IV Cell Invasion Assay TREVIGEN INC. :: 8405 HELGERMAN CT. :: GAITHERSBURG, MD 20877 1.800.TREVIGEN :: 1.800.873.8443 :: (FAX) 1.301.560.4973 :: INFO@TREVIGEN.COM