Shopping Cart » DIVAATM     » Cell Invasion     » PathClear® BME     » PARP     » CometAssayTM     » HT 8-oxo-dG ELISA  Home » Angiogenesis & Cell Differentiation Angiogenesis & Cell Differentiation Cultrex® 3D Culture Matrix™ Laminin I 3-D Culture is an innovative approach to modeling the morphological effects of early oncogenesis on glandular epithelial cells. When healthy, these cells exhibit a structured, polarized morphology that is critical for cellular formation and function. During carcinoma development, cell cycle controls associated with cellular development, proliferation and death are lost, and as a result, these ascinaric structures are disrupted. In effect, the morphology of these structures can be used as a measure to study factors in early carcinoma development. Trevigen has developed the 3D Culture Matrix™ product line to provide reagents specifically produced for and qualified in 3-D culture studies. Applications: The 3-D Culture Matrix™ Laminin I may be used as a gel on which to grow cells or a media additive alone or in concert with other basement membrane components to study cellular growth and differentiation in three dimensions in vitro.   Preparation: 3-D Culture Matrix™ Laminin I is provided in DMEM medium containing 10 µg/ml gentamycin sulfate and no phenol red at a concentration of ~ 3mg/ml.   Source: Purified from murine EHS sarcoma.   Storage: Store at -20° C or -80° C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.   References: Debnath J, Muthuswamy SK, Brugge JS: Morphogenesis and oncogenesis of MCF-10A mammary epithelial acini grown in three-dimensional basement membrane cultures. Methods, 2003 pp. 256-268. Webber MM, Bello D, Kleinman HK, and Hoffman MP. Acinar differentiation by non-malignant immortalized human prostate epithelial cells and its loss in malignant cells. Carcinogenesis. 1997. Jun; 18(6): 1225-1231. Top   Ordering Information Catalog #: Price: 3446-005-01 3D Culture Laminin I, 5 ml $230.00 Trevigen Products are now available for ordering through Download Protocol 3-D Culture MatrixTM Laminin I Related Products Cultrex Basement Membrane Extract Cultrex 3D Culture Matrix BME Cultrex 3D Culture Matrix Rat Collagen I Cultrex 3D Culture Matrix Cell Harvesting Kit Cultrex 3D Culture 96 Well Laminin I Cell Proliferation Assay Cultrex 3D Culture 96 Well BME Cell Proliferation Assay Cultrex 3D Culture 96 Well Collagen I Cell Proliferation Assay Cultrex 3D Culture Cell Proliferation Assay Core Kit TREVIGEN INC. :: 8405 HELGERMAN CT. :: GAITHERSBURG, MD 20877 1.800.TREVIGEN :: 1.800.873.8443 :: (FAX) 1.301.560.4973 :: INFO@TREVIGEN.COM