Shopping Cart » PARP in vivo PDA   » 3D Assays   » Stem Cell Assays   » Cell Invasion   » PathClear® BME   » PARP   » CometAssay®  Home » Angiogenesis & Cell Differentiation Angiogenesis & Cell Differentiation Cultrex® 96 Well BME Cell Invasion Assay Cultrex® Cell Invasion Assays were created in an effort to accelerate the screening process for compounds that influence cell migration through extracellular matrices. These assays offer a flexible, standardized, high throughput format for quantitating the degree to which invasive cells penetrate a barrier consisting of basement membrane components in vitro in response to chemoattractants and/or inhibiting compounds. The Cultrex® Cell Invasion Assay has been adapted to multiple formats so that cell invasion may be evaluated against different extracellular matrices; the assay is available in the following formats: Basement Membrane Extract (BME), Laminin I, Collagen I, Collagen IV. Trevigen’s Cultrex® 96 well Cell Migration Assay utilizes a simplified Boyden chamber design with an 8 mm polyethylene terephthalate (PET) membrane. Ports within the migration chamber (top) allow access to the assay chamber (bottom). The inserts are sufficiently large to allow for subsequent analysis of migrating subpopulations, in response to chemokines, cytokines, drugs or other compounds of interest. Detection of cell invasion is quantified using Calcein-AM. Calcein-AM is internalized, and intracellular esterases cleave the acetomethylester (AM) moiety to generate free Calcein. Free Calcein fluoresces brightly, and this fluorescence may be used to quantitate the number of cells that have migrated using a standard curve. Applications: Cell migration/Invasion Assays   Components: Part # Component Size   3455-096-01 Cell Invasion Chamber each   3455-096-02 5X BME Solution 1 ml   3455-096-03 10X Coating Solution 1 ml   3455-096-04 25X Cell Wash Buffer 2 x 1.5 ml   3455-096-05 10X Cell Dissociation Solution 2 x 1.5 ml   4892-010-01 Calcein AM 50 µg   Storage: Components are stored at -20ºC, 4ºC, and at room temperature.   References: Liotta, L.A. 1984. Tumor invasion and metastasis: role of the basement membrane. Am. J. Pathol. 117:339-348. Terranova, V.P., E.S. Hujanen, D.M. Loeb, G.R. Martin, L. Thornburg, and V. Glushko. 1986. Use of reconstituted basement membrane to measure cell invasiveness and select for highly invasive tumor cells. Proc. Natl. Acad. Sci. USA83:465-469. Albini, A., Y. Iwamoto, H.K. Kleinman, G.R. Martin, G.R. Aaronson, J.M. Kozlowski, and R.N. McEwan. 1987. A rapid in vitroassay for quantitating the invasive potential of tumor cells. Cancer Res. 47:3239-3245. Top   Ordering Information Catalog #: Price: 3455-096-K Cultrex 96 Well BME Cell Invasion Assay, 96 samples $340.00 Trevigen Products are now available for ordering through - AND - Download Protocol Cultrex® 96 Well BME Data Technical Presentations   Related Products Cultrex 96 Well Endothelial Cell Invasion Kit Cultrex 96 Well Collagen I Cell Invasion Assay Cultrex 96 Well Collagen IV Cell Invasion Assay Cultrex 96 Well Laminin I Cell Invasion Assay Cultrex 96 Well Cell Migration Assay Cultrex 24 Well Collagen I Cell Invasion Assay Cultrex 24 Well Laminin I Cell Invasion Assay Cultrex 24 Well Collagen IV Cell Invasion Assay Cultrex 24 Well BME Cell Invasion Assay CultreCoat® 24 Well BME-Coated Cell Invasion Assay Cultrex 24 Well Cell Migration Assay TREVIGEN INC. :: 8405 HELGERMAN CT. :: GAITHERSBURG, MD 20877 1.800.TREVIGEN :: 1.800.873.8443 :: (FAX) 1.301.560.4973 :: INFO@TREVIGEN.COM :: SITE MAP