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Cultrex® Calcein-AM Cell Viability Kit

The Calcein-AM Kit provides a simple, rapid and accurate method to measure cell viability and/or cytotoxicity. Calcein-AM is a non-fluorescent, hydrophobic compound that easily permeates intact, live cells. The hydrolysis of Calcein-AM by intracellular esterases produces calcein, a hydrophilic, strongly fluorescent compound that is well-retained in the cell cytoplasm. Cells grown in black-walled plates can be stained and quantified in less than two hours.

Features:
  • Suitable for proliferating and non-proliferating cells
  • Ideal for both suspension and adherent cells
  • Non-radioactive microplate
  • Rapid (no solubilization step as in an MTT assay)
  • Ideal for high-throughput assays
  • Better retention and brightness compared to other fluorescent compounds (i.e. fluorescein)
  • Useful in a variety of studies, including: cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis, and cytotoxicity
  • Adaptable to a wide variety of techniques, including: microplate assays (described here), immunocytochemistry, flow cytometry, and in vivo cell tracing
  •  
    Applications:
  • Cell viability assays
  • Cytotoxicity analysis
  •  
    Components: Part # Component Size
      4892-010-01 Calcein AM 50 µg
      4892-010-02 10X Calcein AM DW Buffer 200 ml
     
    Storage: Components are stored at -20°C and 4°C.
     
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    Ordering Information
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    Catalog #: Price:

    4892-010-K
    Calcein AM Cell Viability Assay Kit,
    1000 Tests
    $185.00
    Add to Cart

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