| Features: |
Fast. Requires less than 3 hours to complete.
Exclusive, non-toxic TACS Safe TdT buffer - sodium cacodylate free.
Unique buffer system produces more consistent labeling.
Performance tested on heart derived samples.
Exclusive CytoninTM permeabilization reagent included.
TACS-Nuclease solution for preparing sample dependent positive controls included.
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| |
| Applications: |
TUNEL assay
In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cardiac cells and tissues.
Assists in the identification of apoptotic morphologies.
Helps resolve unique problems encountered when detecting apoptotic cardiac cells.
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| Components: |
Part # |
Component |
Size |
| |
4800-30-01 |
Proteinase K |
30 µl |
| |
4800-30-06 |
Strep-HRP |
30 µl |
| |
4800-30-11 |
TACS Blue Label™ |
3 ml |
| |
4800-30-12 |
Blue Strep-HRP Diluent |
7.5 ml |
| |
4800-30-14 |
Streptavidin-FITC |
30 µl |
| |
4800-30-15 |
TACS-Nuclease™ |
15 µl |
| |
4800-30-16 |
TACS-Nuclease Buffer |
15 µl |
| |
4800-30-17 |
Nuclear Fast Red |
50 ml |
| |
4810-30-02 |
TACS 2 TdT Labeling Buffer |
100 ml |
| |
4810-30-03 |
TACS 2 TdT Stop Buffer |
100 ml |
| |
4810-30-04 |
TACS 2 TdT dNTP |
30 µl |
| |
4810-30-05 |
TdT Enzyme |
30 µl |
| |
4876-05-01 |
Cytonin™ |
5 ml |
| |
|
| |
| Storage: |
Components are stored at -20°C, 4°C, and room temperature.
|
| |
| References: |
1. J. F. Kerr, G. C. Gobe, C. M. Winterford and B. V. Harmon (1995) Anatomical methods in cell death. Methods in Cell Biology 46, 1 - 27.
2. M. Yamawaki, A. Zurbriggen, A. Richard and M. Vandevelde (1993) Saponin treatment for in situ hybridization maintains good morphological preservation. J. Histochem. Cytochem. 41:105 - 109.
3. S.-R. Shi, R.J. Cote, L.L. Young and C.R. Taylor (1997) Antigen retrieval immunohistochemistry: practice and development. J. Histotechnology 20:145 - 154.
4. A. Migheli, A. Attanasio, W.-H. Lee, S.A. Bayer and B. Ghetti (1995) Detection of apoptosis in weaver cerebellum by electron microscopic in situ end-labeling of fragmented DNA. Neurosci. Lett. 199:53 -56.
|
kit is based on DNA end-labeling using terminal deoxynucleotidyl
transferase (TdT) and a modified nucleotide that is subsequently
detected using our TACS Blue Label™ detection system.
Trevigen has developed an exclusive apoptosis grade TdT enzyme
that coupled with the TACS Blue Label solution provides labeling
20 to 50 times more sensitive than standard diaminobenzidine
(DAB) labeling methods. To ensure ease of data interpretation
when the numbers of apoptotic cells are low, CardioTACS provides
Nuclear Fast Red Counterstain which provides superb contrast
to the TACS Blue Label in apoptotic cells. In addition, the
kit includes TACS-Nuclease™ that is used to generate
a positive control in your own sample. The positive control
provides an internal control for permeabilization and labeling
so optimization is nominal and the data can be interpreted
with confidence.
