| Features: |
Fast. Requires less than 3 hours to complete.
Exclusive, non-toxic TACS Safe TdTTM buffer - sodium cacodylate free.
Unique buffer system produces more consistent labeling.
Performance tested on tumor samples.
Includes exclusive CytoninTM permeabilization reagent.
Includes TACS-Nuclease solution for preparing sample-dependent positive controls.
Includes DAB Enhancer for intensifying and darkening DAB staining.
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| Applications: |
In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.
Assists in the identification of apoptotic morphologies.
Helps resolve unique problems encountered when using tissues or cells from tumors.
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| Components: |
Permeabilization reagents
Labeling enzyme
Chromogenic substrate
Labeling buffer
Optimized cation
Counterstain
Nucleotide mix
Detection reagents
DAB Enhancer
TACS-Nuclease
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| Items Not Included: |
Reagents for dehydration and rehydration, H2O2, PBS buffer, and disposables.
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| Storage: |
Components are stored at -20°C, 4°C, and room temperature. |
incorporated
nucleotides are subsequently detected using a horseradish
peroxidase conjugate. The conjugate catalyzes the conversion
of diaminobenzidine (DAB) into a visual dark brown precipitate.
The kit includes methyl green counterstain which is a general
counterstain appropriate for a wide variety of tissues and
tumors types and provides good contrast with the brown DAB
in labeled cells. TumorTACS contains all the reagents required
for permeabilizing, labeling, staining and counterstaining
tumor samples, including TACS-Nuclease™ for preparing
positive controls.
