Trevigen is pleased to provide highly qualified enzymes, antibodies, and assay kits for DNA damage and repair research. As always, our products are accompanied by detailed product data sheets or instructions for use, and backed with expert technical support.
DNA repair pathways maintain the integrity of the genome reducing the onset of cancer, disease and aging phenotypes. To further study repair pathways, Trevigen offers panels of human cell lines each deficient in proteins associated with DNA repair. This consists of cell lines deficient for proteins in the Base Excision Repair Pathway, Non Homologous End Joining, Homologous Recombination and Mismatch Repair. For the availability of other DNA repair deficient cell lines contact Trevigen.
PARP-1 contributes to the sequence of events that occurs during DNA base excision repair. Trevigen offers kits that measure the in vivo and in vitro activities of PARP and PARG.
The single cell gel electrophoresis or CometAssay® is a state-of-the-art technique for quantitating DNA damage and repair from in vivo and in vitro samples of eukaryotic cells and some prokaryotic cells. In conjunction with Trevigen’s new CometAssay® Electrophoresis System, which eliminates known causes of assay variability, it is the only technique that directly measures DNA damage in individual cells and as a result has rapidly gained importance in the fields of genetic toxicology and human biomonitoring.
Trevigen’s unique FLARE™ Assays characterize DNA damage in single cells using a variety of DNA repair enzymes that recognize oxidative and UV damage in conjunction with Trevigen’s CometAssay® Electrophoresis System. A variety of DNA repair enzymes and antibodies are available as individual components with optimized assay conditions and buffers for research in areas of double-strand break repair, base excision repair, DNA methylation, oxidative damage, UV damage, and radiation damage.