Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma. Basement membranes are degraded and regenerated during development and wound repair. They not only support cells and cell layers, but they also play an essential role in tissue organization that affects cell adhesion, migration, proliferation, and differentiation. Basement membranes provide major barriers to invasion by metastatic tumor cells.
Cultrex® Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. The extract gels at 37°C to form a reconstituted basement membrane. The major components of BME include laminin, collagen IV, entactin, andheparin sulfate proteoglycan. Trevigen's BME typically ranges in protein concentrations from 14 to 16 µg/ml. BME is commonly used to maintain stem and precursor cells in an undifferentiated state during cell culture and BME hydrogels function to induce differentiation of epithelial, endothelial and smooth muscle cells in vitro. Cultrex® BME is also available in PathClear® and HC20+ varieties. BME has also been employed in cell attachment, neurite outgrowth, angiogenesis, and in vitro cell invasion assays. BME is available with, and without Phenol Red.
The PathClear® designation for EHS derived material means that in addition to standard sterility, endotoxin and MAP testing, the basement membrane extract is tested by PCR and is clear of 31 pathogens and viruses, including lactate dehydrogenase elevating virus (LDEV), which replicates in macrophages. Each lot is rigorously qualified in biological performance assays. Trevigens BME typically ranges in protein concentrations from 14 to 16 µg/ml. PathClear® BME is available in normal and reduced growth factor form, with and without phenol red, and is ideal for in vivo murine research work and other work requiring BME free from viruses, bacteria and mycoplasma.
Cultrex® PathClear® HC20+ was developed for use in in vivo applications such as tumorigenicity assays, where higher protein concentrations facilitate faster gelling times, increased gel strength, and elevated levels of tumor augmentation. Many primary tumor cells that will not proliferate in vitro, will proliferate as a xenograft when embedded in Cultrex® PathClear® HC20+ basement membrane extract.
Cultrex® 3-D Culture Matrix™ BME is prequalified for its ability to support the culture of cells in three dimensions. 3D culture is an innovative approach to modeling the morphological effects of early oncogenesis on glandular epithelial cells. When healthy, these cells exhibit a structured, polarized morphology that is critical for tissue architecture and function. During carcinoma development, cell cycle controls associated with cellular development, proliferation and death are lost, and as a result, acinar structure formation is disrupted. In effect, the morphology of these structures can be used as a measure to study factors in early carcinoma development. Trevigens BME typically ranges in protein concentrations from 14 to 16 µg/ml.
Phenol Red:
- Phenol red (catalog # 3430-50-01), provided separately at 100X needs to be added at 1:100 (v/v) prior to use.
Shared Specifications:
- Endotoxin (LPS) Concentration: ≤ 8 EU/ml
- Source: Murine Engelbreth-Holm-Swarm (EHS) tumor.
- Storage Buffer: Dulbecco’s Modified Eagle’s medium containing 10 µg/ml gentamycin sulfate +/- phenol red.
- Trevgens BME typically ranges in protein concentrations from 14 to 16 µg/ml.
- Protein concentrations over 20 µg/ml are available in HC20+.
- Storage/Stability: Product is stable for a minimum of 3 months from date of shipment when stored at –20 °C in a manual defrost freezer. For optimal stability, store at –80 °C in aliquots. Keep Frozen; repeated freeze-thaws will destroy product integrity.
Cultrex® Basement Membrane Extract and Specialty Proteins (Human) | ||||
| Catalog # | Product Name | Size | Price | |
| 3415-001-02 | Cultrex® Human BME PathClear® | 1ml | $175.00 | |
| 3420-001-01 | Cultrex® Human Fibronectin, PathClear® | 1 mg | $95.00 | |
| 3421-001-01 | Cultrex® Human Vitronectin, PathClear® | 50 µg | $95.00 | |
| 3422-001-01 | Cultrex® Human Vitronectin, Nucleic Acids Reduced, PathClear® | 50 µg | $150.00 | |
Cultrex® BME PathClear® (PC) | ||||
| Catalog # | Product Name | Size | Price | |
| 3430-001-02 | Cultrex® BME with phenol red, PathClear® | 1 ml | $40.00 | |
| 3430-005-02 | Cultrex® BME with phenol red PathClear® | 5 ml | $160.00 | |
| 3431-001-02 | Cultrex® BMEwith phenol red, reduced growth factor, PathClear® | 1 ml | $40.00 | |
| 3431-005-02 | Cultrex® BME with phenol red, reduced growth factor, PathClear® | 5 ml | $190.00 | |
| 3432-001-02 | Cultrex®BME, without phenol red, PathClear® | 1 ml | $40.00 | |
| 3432-005-02 | Cultrex® BME, without phenol red, PathClear® | 5 ml | $160.00 | |
| 3433-001-02 | Cultrex® BME, without phenol red, reduced growth factor, PathClear® | 1 ml | $40.00 | |
| 3433-005-02 | Cultrex® BME, without phenol red reduced growth factor, PathClear® | 5 ml | $190.00 | |
| 3444-010-02 | Cultrex® High Protein Concentration (HC20+) BME, PathClear® | 2 X 5 ml | $350.00 | |
| 3430-010-02 | Cultrex® BME with phenol red PathClear® | 2 X 5 ml | $225.00 | |
| 3432-010-02 | Cultrex® BME, without phenol red, PathClear® | 2 X 5 ml | $225.00 | |
| 3431-010-02 | Cultrex® BME with phenol red, reduced growth factor, PathClear® | 2 X 5 ml | $275.00 | |
Cultrex® BME High Protein Concentration (HC20+) | ||||
| Catalog # | Product Name | Size | Price | |
| 3444-005-02 | Cultrex® High Protein Concentration (HC20+) BME, PathClear® | 5ml | $205.00 | |
Cultrex® BME Stem Cell Qualified | ||||
| Catalog # | Product Name | Size | Price | |
| 3434-005-02 | Cultrex® Stem Cell Qualified, BME Growth Factor Reduced PathClear® | 5 ml | $210.00 | |
| 3420-001-03 | Cultrex® Stem Cell Qualified Human Fibronectin, PathClear® | 1 mg | $110.00 | |
| 3421-001-03 | Cultrex® Stem Cell Qualified Human Vitronectin, PathClear® | 200 µg | $245.00 | |
Cultrex® BME 3-D Culture™ Matrix | ||||
| Catalog # | Product Name | Size | Price | |
| 3445-001-01 | 3-D Culture Matrix | 1 ml | $50.00 | |
| 3445-005-01 | 3-D Culture Matrix | 5 ml | $215.00 | |
| 3445-010-01 | 3-D Culture Matrix | 2 X 5 ml | $330.00 | |
Trevigen Exclusive
