CometAssay®
The single cell gel electrophoresis or CometAssay® is a state-of-the-art technique for quantitating DNA damage and repair from in vivo and in vitro samples of eukaryotic cells and some prokaryotic cells. In conjunction with Trevigen's new CometAssay® Electrophoresis System, which eliminates known causes of assay variability, it is the only technique that directly measures DNA damage in individual cells and as a result has rapidly gained importance in the fields of genetic toxicology and human biomonitoring.
- Cells mixed with low melting agarose at 37oC (LM Agarose)
- Immobilize cells on CometSlide™
- Treat cells with Lysis Solution (removes membranes and histones from the DNA)
- Samples treated with alkali (unwinds and denatures DNA)
- Samples stained with intercalating dye and visualized by epifluorescence microscopy following alkaline electrophoresis, which reveals DNA breaks.
CometAssay® Electrophoresis Systems | ||||
| Catalog # | Product Name | Size | Price | |
| 4250-050-ES | CometAssay® Electrophoresis System | 1 system | $1995.00 | |
| 4250-050-ESK | CometAssay® 2 Well ES Unit w/ Starter Kit | 1 system, 1 starter kit | $2115.00 | |
| 4252-040-ESK | CometAssay® 20 Well ES Unit w/ Starter Kit | 1 system,1 starter kit | $2115.00 | |
| 4252-040-ESK-PS1 | CometAssay® 20 Well ES Unit w/ Starter Kit plus Power Supply for North America | 1 system, 1 starter kit, 1 power supply | $2565.00 | |
| 4253-096-ESK | CometAssay® 96 Well ES Unit w/ Starter Kit | 1 system, 1 stater kit | $2115.00 | |
| 4253-096-ESK-PS1 | CometAssay® 96 Well ES Unit w/ Starter Kit plus Power Supply for North America | 1 system, 1 starter kit, 1 power supply | $2565.00 | |
| 4250-050-ESK-PS1 | CometAssay® 2 Well ES Unit w/ Starter Kit and Power Supply | 1 system, 1 starter kit, 1 power supply | $2565.00 | |
CometAssay® Reagent Starter Kits | ||||
| Catalog # | Product Name | Size | Price | |
| 4250-050-K | CometAssay® Kit 50 Samples ( 25X2 well slides) | 50 samples | $225.00 | |
| 4252-040-K | CometAssay® Kit, 40 Samples (2X20 well slides) | 40 samples | $225.00 | |
| 4253-096-K | CometAssay® Kit 96 Samples (1X96) | 96 samples | $225.00 | |
CometAssay® Reagents | ||||
| Catalog # | Product Name | Size | Price | |
| 4250-050-01 | CometAssay® Lysis Solution | 2 x 500 ml | $70.00 | |
| 4250-050-02 | CometAssay® LM Agarose | 15 ml | $40.00 | |
| 4250-050-05 | SYBR Green | 5 μl | $25.00 | |
CometAssay®Control Cells | ||||
| Catalog # | Product Name | Size | Price | |
| 4256-010-CC | CometAssay® Alkaline Control Cells | 10 assays | $130.00 | |
| 4257-010-NC | CometAssay® Neutral Control Cells | 10 assays | $130.00 | |
Silver Staining Kit | ||||
| Catalog # | Product Name | Size | Price | |
| 4251-050-K | CometAssay® Silver (CometAssay Kit w/silver staining reagents) | 50 samples | $315.00 | |
| 4254-200-K | CometAssay® Silver Staining Kit | 200 samples | $160.00 | |
FLARE™ Assay Kits and Modules | ||||
| Catalog # | Product Name | Size | Price | |
| 3950-075-SP | FLARE™ Sample Prep | 75 tests | $230.00 | |
| 4040-100-FK | Fpg FLARE™ Assay Kit | 75 samples | $340.00 | |
| 4040-100-FM | Fpg FLARE™ Module | >100 samples | $315.00 | |
| 4045-01K-FK | E. coli Endonuclease III FLARE™ Kit | >75 samples | $340.00 | |
| 4045-01K-FM | E. coli Endonuclease III FLARE™ Module | >100 samples | $315.00 | |
| 4055-100-FK | T4-PDG FLARE™ Assay Kit | 75 samples | $340.00 | |
| 4055-100-FM | T4-PDG FLARE™ Module | >100 samples | $315.00 | |
| 4065-100-FK | cv-PDG FLARE™ Assay Kit | 75 samples | $340.00 | |
| 4065-100-FM | cv-PDG FLARE™ Module | >100 samples | $315.00 | |
| 4100-100-FK | UVDE FLARE™ Assay Kit | 75 samples | $340.00 | |
| 4100-100-FM | UVDE FLARE™ Module | >100 samples | $315.00 | |
| 4130-100-FK | hOGG1 FLARE™ Assay Kit | 75 samples | $340.00 | |
| 4130-100-FM | hOGG1 FLARE™ Module | 100 samples | $315.00 | |
Slides and Rack System | ||||
| Catalog # | Product Name | Size | Price | |
| 3950-075-02 | FLARE™ Slides (3 Well, 25 Slides) | 25 slides | $130.00 | |
| 3950-300-02 | FLARE™ Slides (3 Well, 100 Slides) | 100 slides | $290.00 | |
| 4250-004-03 | CometSlide™ (2 Well, 2 Slides) | 2 slides | $35.00 | |
| 4250-050-03 | CometSlides™ (2 Well, 25, Slides) | 25 each | $90.00 | |
| 4250-200-03 | CometSlides™ (2 Well, 100 Slides) | 100 slides | $225.00 | |
| 4252-02K-01 | CometAssay® HT Slides (20 Well, 100 Slides) | 100 slides | $975.00 | |
| 4252-040-01 | CometAssay® HT Slides (20 Well, 2 Slides) | 2 slides | $45.00 | |
| 4252-040-02 | CometSlide™ Rack System | each | $90.00 | |
| 4252-200-01 | CometAssay® HT (20 Well, 10 Slides) | 10 slides | $185.00 | |
| 4252-500-01 | CometAssay® HT (20 Well, 25 Slides) | 25 slides | $315.00 | |
| 4253-096-03 | CometSlide™ (96 Well) | 1 slide | $45.00 | |
Trevigen Exclusive