| Features: |
Colorimetric format
High throughput 96 test size
Standard curve has a range of 0.94 - 60 ng/ml
Sensitive to 0.59 ng/ml
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| Applications: |
For the detection and quantification of 8-hydroxy-2’-deoxyguanosine, 8-hydroxyguanosine, 8-hydroxiguanine, guanosine, 8-bromoguanosine, 2’-deoxyinosine, 8-mercaptoguanosine, and N2-methylguanosine in urine, serum and saliva samples. |
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| Components: |
Part # |
Component |
Size |
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4370-096-01 |
8-oxo-dG Immunoassay Plate |
96 well plate |
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4370-096-02 |
8-oxo-dG Standard |
25 µL |
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4370-096-03 |
Sample Diluent |
500 ml |
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4370-096-04 |
20x Wash Buffer |
100 ml |
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4370-096-05 |
Anti-8-oxo-dG |
25 µL |
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4370-096-06 |
Antibody Diluent |
6 ml |
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4370-096-07 |
Anti-Mouse IgG HRP Conjugate |
25 µL |
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4370-096-08 |
HRP Conjugate Diluent |
12 ml |
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4370-096-09 |
TMB Substrate |
12 ml |
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4370-096-10 |
Stop Solution 2 |
11 ml |
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| Storage: |
All reagents are stable as supplied at 4°C, except the 8-oxo-dG Standard, which should be stored at -20°C. For optimum storage, the 8-oxo-dG Standard should be aliquotted into smaller portions and stored at -20°C to avoid repeated freeze/ thaw cycles.
Unused wells of the 8-oxo-dG Immunoassay Plate should be resealed with desiccant in the foil pouch provided and stored at 4°C until the kit expiration date.
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| References: |
Evans, M. D., Dizdaroglu, M., and Cooke, M. S. (2004) Mutat. Res. 567: 1-61.
Sancar, A., Lindsey-Boltz, L. A., Unsal-Kacmaz, K., and Linn, S. (2004) Annu. Rev. Biochem.73: 39-85.
Chiou, C. C., Chang, P. Y., Chan, E. C., Wu, T. L., Tsao, K. C., and Wu, J. T. (2003) Clin.Chim. Acta. 334: 87-94.
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