Shopping Cart » DIVAATM     » Cell Invasion     » PathClear® BME     » PARP     » CometAssayTM     » HT 8-oxo-dG ELISA  Home » DNA Damage DNA Damage HT Colorimetric PARG Assay Kit Poly(ADP-ribose) glycohydrolase (PARG) degrades poly(ADP-ribose) (PAR) polymers synthesized by poly(ADP-ribose) polymerase (PARP1). When activated by DNA strand breaks, PARP1 uses NAD as a substrate to form ADP-ribose polymers on itself and on specific acceptor proteins such as histones, DNA polymerases, DNA ligases, p53, and Fos. These polymers are in turn rapidly degraded by PARG, a ubiquitously expressed exo- and endoglycohydrolase. Excessive activation of PARP1 leads to NAD depletion and cell death during ischemia and other conditions that generate extensive DNA damage. PARG may maintain the active state of PARP1 by continuously removing inhibitory ADP-ribose residues from PARP1.1 The regulation of PARG activity may therefore, influence PARP-mediated cell death. First, PARG inhibition could slow the turnover of PAR and thereby limit NAD depletion. Second, PARG inhibition could prevent the removal of PAR from PARP1. Because PARP1 is inhibited by extensive poly-(ADP-ribosyl)ation, PARG inhibitors could thereby indirectly inhibit PARP1 activity.2 Prior work has shown that the PARG inhibitor gallotannin can markedly reduce death of astrocytes after oxidative stress. Trevigen’s HT Colorimetric PARG Assay Kit measures the loss of biotinylated PAR from histones attached to Strip Wells in a 96 well format and is ideal for the screening of PARG inhibitors and for measuring the activity of PARG in cell extracts.¿ Features: Colorimetric, non-radioactive format High throughput 96 test size Sensitivity down to 50 pg of PARG per well   Applications: Identify inhibitors and activators of PARG activity Measuring activity of PARG in cell and tissue extracts   Components: Part # Component Size   4671-096-02 *20X PARP Buffer 500 µl   4671-096-03 *10X PARP Cocktail** 300 µl   4671-096-06 *10X Activated DNA 300 µl   4677-096-P *Histone-Coated Strip Wells (natural) 96 wells   4680-096-01 *PARG Enzyme (1µg/mL) 100 µl   4680-096-02 *10X PARG Buffer 8 mL   4680-096-03 *DEA, 100 mM 200 µl   4680-096-04 Strep-HRP80 30 µL   4822-96-08 TACS-Sapphire™ 10 ml   *Components marked with an asterisk can be stored at -20 C for one year. **Coated with biotinylated PAR   Storage: Components are stored at -80°C and 4°C.   References: Ying W, and Swanson RA. 2000. The poly(ADP-ribose) glycohydrolase inhibitor gallotannin blocks oxidative astrocyte death. Neuroreport. 11:1385-1388. Oei SL, Keil C, Ziegler M. 2005. Poly(ADP-ribosylation) and genomic stability. Biochem Cell Biol. 83:263-269. Top   Ordering Information Catalog #: Price: 4683-096-K HT Colorimetric PARG Assay Kit, 96 Tests $485.00 Trevigen Products are now available for ordering through Download Protocol Related Products HT Chemiluminescent PARG Assay Kit HT Colorimetric PARP/Apoptosis Assay Human PARP Enzyme (Standard) HT Fluorescent Homogeneous PARP Inhibition Assay HT Chemiluminescent PARP/Apoptosis Assay Anti-PAR Polymer Monoclonal Affinity Purified Antibody Anti-PAR Polymer Polyclonal Antibody Anti-PARP Monoclonal Antibody Human PARP Enzyme (HSA) High Specific Activity Biotinylated NAD FITC-NAD Universal Colorimetric PARP Assay Kit Universal Chemiluminescent PARP Assay Kit PARP Inhibitors Anti-PAR Polymer Monoclonal Antibody TREVIGEN INC. :: 8405 HELGERMAN CT. :: GAITHERSBURG, MD 20877 1.800.TREVIGEN :: 1.800.873.8443 :: (FAX) 1.301.560.4973 :: INFO@TREVIGEN.COM