CometAssayTM Electrophoresis System
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The CometAssayTM Electrophoresis System and CometAssayTM Control cells (cat# 4256-010-CC) enable investigators to consistently optimize alkaline comet assays for highly reproducible results, and to standardize alkaline electrophoresis methods between individual users and laboratories. The comet assay is the only direct method for the detection of DNA damage in cells. It is used in cancer research, in genotoxicity studies on environmental mutagens, and for screening compounds for cancer therapeutics. The Comet Assay can be variable depending on temperature, distance between electrodes, and buffer height. Trevigen, with funding from Phase II SBIR grants**, has solved these problems and makes available a novel complete assay system which includes CometSlidesTM, reagents, control cells and an electrophoresis unit which retains test cells in a uniquely configured electrophoretic field permitting consistent DNA migration patterns, which are critical for standardization of the assay. Each lot of the CometAssayTM control cells, reagents and CometSlidesTM developed by Trevigen are tested and qualified for use in the CometAssayTM System.
**The project described was supported by Grant Number R44CA096390 from the National Cancer Institute. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Cancer Institute or the National Institutes of Health.
CometAssay Electrophoresis System Starter Kit (4250-050-ESK) includes: the electrophoresis system 4250-050-ES, a CometAssayTM Kit 4250-050-K and CometAssayTM Control Cells 4256-010-CC ( choice of: 2 well CometSlides, 20 well CometSlides, 96 well CometSlides).
UK Verision: POWER SUPPLY 230V UK CS Cat #: 33500-170
VWR: PowerSourceTM 300V power supply Cat# 93000-744.
** Power Supply: Power Source™ 300V by VWR. The CometAssay Electrophoresis System has been "used" / "qualified" with the VWR Power Source power.
| Applications: |
An acrylic overlay on top of the elevated slide tray maintains optimal buffer height for DNA migration.
A constant buffer temperature is maintained using a water chamber to cool the ceramic slide platform and buffer chamber.
Smoke grey colored unit minimizes exposure to UV light.
Specially designed slide trays accommodate 2, 20 and 96 well slides and maintain proper slide orientation during electrophoresis.
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| References: |
1. Lemay, M. and K.A. Wood, 1999. Detection of DNA damage and identification of UVinduced photoproducts using the CometAssayTM kit. BioTechniques 27(4):846-851.
2. Angelis, K.J., M. Dusinska and A.R. Collins. 1999. Single cell gel electrophoresis:Detection of DNA damage at different levels of sensitivity. Electrophoresis 20:2133-2138.
3. Morris, E.J., J.C. Dreixler, K-Y. Cheng, P.M. Wilson, R.M. Gin and H.M. Geller. 1999. Optimization of single-cell gel electrophoresis (SCGE) for quantitative analysis of neuronal DNA damage. BioTechniques 26:282-289.
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Ordering Information
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Catalog #:
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Price:
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4250-050-ESK
CometAssay Electrophoresis System Starter Kit,
1 Unit, 1 Kit, 1 Set |
$2,115.00
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4250-050-ES
CometAssay Electrophoresis System,
1 Unit |
$1,995.00
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Download Protocol
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