CometAssay® Electrophoresis System
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The CometAssay® Electrophoresis System and CometAssay® Control cells (cat# 4256-010-CC) enable investigators to consistently optimize alkaline comet assays for highly reproducible results, and to standardize alkaline electrophoresis methods between individual users and laboratories. The comet assay is the only direct method for the detection of DNA damage in cells. It is used in cancer research, in genotoxicity studies on environmental mutagens, and for screening compounds for cancer therapeutics. The Comet Assay can be variable depending on temperature, distance between electrodes, and buffer height. Trevigen, with funding from Phase II SBIR grants**, has solved these problems and makes available a novel complete assay system which includes CometSlides®, reagents, control cells and an electrophoresis unit which retains test cells in a uniquely configured electrophoretic field permitting consistent DNA migration patterns, which are critical for standardization of the assay. Each lot of the
® control cells, reagents and CometSlides® developed by Trevigen are tested and qualified for use in the CometAssay® System.
**The project described was supported by Grant Number R44CA096390 from the National Cancer Institute. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Cancer Institute or the National Institutes of Health.
| Applications: |
An acrylic overlay on top of the elevated slide tray maintains optimal buffer height for DNA migration.
A constant buffer temperature is maintained using a water chamber to cool the tempered glass slide platform and buffer chamber.
Smoke grey colored unit minimizes exposure to UV light.
Specially designed slide trays accommodate 2, 20 and 96 well slides and maintain proper slide orientation during electrophoresis.
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| Components: |
Part # |
Component |
Size |
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4250-050-ES |
CometAssay Electrophoresis System |
1 Unit |
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4250-050-K |
CometAssay® Kit |
1 Kit |
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| References: |
1. Lemay, M. and K.A. Wood, 1999. Detection of DNA damage and identification of UVinduced photoproducts using the CometAssay® kit. BioTechniques 27(4):846-851.
2. Angelis, K.J., M. Dusinska and A.R. Collins. 1999. Single cell gel electrophoresis:Detection of DNA damage at different levels of sensitivity. Electrophoresis 20:2133-2138.
3. Morris, E.J., J.C. Dreixler, K-Y. Cheng, P.M. Wilson, R.M. Gin and H.M. Geller. 1999. Optimization of single-cell gel electrophoresis (SCGE) for quantitative analysis of neuronal DNA damage. BioTechniques 26:282-289.
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Ordering Information
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Catalog #:
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Price:
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4250-050-ESK
CometAssay 2 Well ES Starter Kit,
1 Unit, 1 Kit, 1 Set |
$2,115.00
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4252-040-ESK
CometAssay 20 Well ES Starter Kit,
1 Unit, 1 Kit, 1 Set |
$2,115.00
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4253-096-ESK
CometAssay 96 Well ES Starter Kit,
1 Unit, 1 Kit, 1 Set |
$2,115.00
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4253-096-ESK-PS1
CometAssay 96 Well ES Starter Kit with Power Supply (North Amer),
1 Unit, 1 Kit, 1 Set |
$2,565.00
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4252-040-ESK-PS1
CometAssay 20 Well ES Starter Kit with Power Supply (North Amer),
1 Unit, 1 Kit, 1 Set |
$2,565.00
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4250-050-ESK-PS1
CometAssay 2 Well ES Starter Kit with Power Supply (North Amer),
I Unit, 1 Kit, 1 Set |
$2,565.00
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