Shopping Cart » DIVAATM     » Cell Invasion     » PathClear® BME     » PARP     » CometAssayTM     » HT 8-oxo-dG ELISA  Home » DNA Damage DNA Damage DASHTM (Diffusion Apoptosis Slide Halo) Assay Kit Trevigen's new CometAssay Kit, DASH™ (Diffusion Apoptosis Slide Halo), allows discrimination of healthy and damaged cells in just a few hours. Simply embed cells in low melting agarose on a pre-treated slide, lyse under alkaline conditions, and precipitate the DNA of the remaining nuclear region (i.e. nucleoid) in the agarose. The precipitated DNA is visualized using **SYBR® Green I nucleic acid stain. When excited (425 - 500 nm) the DNA-bound **SYBR® Green I emits green light. In healthy cells, the fluorescence is confined to the nucleoid: undamaged DNA is supercoiled and thus does not diffuse very far, if at all, from the nucleoid. In cells that have accrued damage to the DNA, the alkali lysis treatment unwinds the DNA, releasing fragments that diffuse away from the center of the nucleoid, creating a "halo" surrounding a small compact origin. This kit includes ready to use, pre-coated slides and optimized reagents for your convenience. Features: Hydrophobic barrier allows easy application of cells directly to slide, and treatment with any of several Trevigen DNA repair enzymes. Easy to use kit format. Work with a small number of cells.   Applications: Detect and quantitate DNA damage Identify apoptotic cells quickly and easily Follow DNA repair   Components: Part # Component Size   4250-050-03 CometSlide (2 well slide) 25 each   4250-050-05 SYBR Green 5 µl   4255-050-01 Lysis solution-DQ 2 x 500 ml   4255-050-02 LMAgarose-DQ 15 ml   4255-050-04 100X NP Buffer-DQ 20 ml   Storage: Components are stored at -20°C or room temperature.   References: Singh NP. (2000) A simple method for accurate estimation of apoptotic cells. Exp Cell Res. 256(1): 328-37 Singh NP, Muller CH, Berger RE. (2003) Effects of age on DNA double-strand breaks and apoptosis in numan sperm. Fertil, Steril. 80(6): 1420-30. Top   Ordering Information Catalog #: Price: 4255-050-K DASH Kit, 50 Samples $225.00 Trevigen Products are now available for ordering through DASH Assay Click the image for more information Download Protocol Related Products CometAssay Kit Higher Throughput CometAssay CometAssay 96 CometAssay Silver Staining Kit TREVIGEN INC. :: 8405 HELGERMAN CT. :: GAITHERSBURG, MD 20877 1.800.TREVIGEN :: 1.800.873.8443 :: (FAX) 1.301.560.4973 :: INFO@TREVIGEN.COM