Shopping Cart » DIVAATM     » Cell Invasion     » Cell Migration     » PARP     » CometAssayTM     » HT 8-oxo-dG ELISA  Home » DNA Damage DNA Damage CometAssayTM Kit Trevigen’s CometAssay™ provides reagents and our exclusive CometSlide™ for the rapid analysis of DNA fragmentation associated with DNA damage. The comet assay or single cell gel electrophoresis assay is based on the alkaline lysis of labile DNA at sites of damage. The unwound, relaxed DNA is able to migrate out of the cell during electrophoresis and can be visualized using **SYBR® Green I nucleic acid gel stain. Cells that have accumulated DNA damage appear as fluorescent comets with tails of DNA fragmentation or unwinding, whereas, normal undamaged DNA does not migrate far from the origin. The CometAssay is provided with our exclusive CometSlides™ which greatly simplify the assay. Each slide provides two sample surfaces specially treated to promote agarose adherence, and a hydrophobic barrier to allow treatment with one of Trevigen’s DNA repair enzymes. Simply add your cells to the low melting point Comet LMAgarose, and pipet onto the slide. Features: CometSlides™ allow direct application of Comet LMAgarose without base layers or pretreatment. Hydrophobic barrier allows easy application of cells directly to slide, and treatment with any of several Trevigen DNA repair enzymes. Easy to use kit format.   Applications: Detect and quantitate DNA damage Identify apoptotic cells quickly and easily Follow DNA repair   Components: Part # Component Size   4250-050-01 CometAssay Lysis Solution 2 x 500 ml   4250-050-02 CometAssay LMAgarose 15 ml   4250-050-03 CometSlide (2 well slide) 25 each   4250-050-04 200 mM EDTA; pH 10 12.5 ml   4250-050-05 SYBR Green 5 µl     Storage: Components are stored at -20°C or room temperature. Top   Ordering Information Catalog #: Price: 4250-050-K CometAssay Kit, 50 Samples $215.00 Trevigen Products are now available for ordering through Download Protocol Related Products CometAssay Control Cells CometAssay 96 FLARE Assay Kits cv-PDG FLARE Assay Kit and Module DASH (Diffusion Apoptosis Slide Halo) Assay Kit hOGG1 FLARE Assay Kit and Module CometAssay Silver Staining Kit CometSlide Fpg FLARE Assay Kit and Module UVDE FLARE Assay Kit and Module CometSlide Rack System T4-PDG FLARE Assay Kit and Module Higher Throughput CometAssay Endonuclease III FLARE Assay Kit and Module A B Click the images below to download PDF files CometAssay™ Poster AACR University of Leicester   CometAssay™ Publication British Journal of Cancer   CometAssay™ Brochure   TREVIGEN INC. :: 8405 HELGERMAN CT. :: GAITHERSBURG, MD 20877 1.800.TREVIGEN :: 1.800.873.8443 :: (FAX) 1.301.560.4973 :: INFO@TREVIGEN.COM