Higher Throughput CometAssayTM
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Trevigen’s CometSlide™ HT provides a convenient
higher throughput, simple and reliable sample preparation,
processing, analysis and storage format for the comet assay.
Trevigen’s CometAssay™ uses our exclusive CometSlide™
HT that is specially treated to promote adherence of low melting
point agarose. This eliminates the time consuming and unreliable
traditional method of preparing base layers of agarose. The
use of CometSlide™ HT shortens assay time, and allows
the rapid and reliable analysis of large numbers of samples.
Trevigen’s CometAssay™ provides
reagents and our exclusive CometSlide™ for the rapid
analysis of DNA fragmentation associated with DNA damage.
The comet assay or single cell gel electrophoresis assay is
based on the alkaline lysis of labile DNA at sites of damage.
The unwound, relaxed DNA is able to migrate out of the cell
during electrophoresis and can be visualized by **SYBR®
Green I nucleic acid gel stain. Cells that have accumulated
DNA damage appear as fluorescent comets with tails of DNA
fragmentation or unwinding, whereas, normal undamaged DNA
does not migrate far from the origin. The CometAssay™ is provided
with our exclusive CometSlides™ HT which greatly simplify the
assay. Each slide provides 20 sample surfaces specially treated
to promote agarose adherence, and a hydrophobic barrier to
allow treatment with one of Trevigen’s DNA repair enzymes.
Simply add your cells to the low melting point Comet LMAgarose,
and pipet onto the slide.
| Features: |
Allows the rapid and reliable analysis of large numbers of samples.
CometSlidesTM allow direct application of Comet LMAgarose without base layers or pretreatment.
Hydrophobic barrier allows easy application of cells directly to slide, and treatment with any of several Trevigen DNA repair enzymes.
Easy to use kit format.
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| Applications: |
For use with Trevigen’s CometAssayTM and FLARE assay reagents and protocol
Detect and quantitate DNA damage
Identify apoptotic cells quickly and easily
Follow DNA repair
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| Storage: |
Store at room temperature with desiccant.
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