| Applications: |
Activity measurements for NAD requiring enzymes
Assays to identify inhibitors of NAD-requiring enzymes
Cell Permeabilization reagent, 4674-250-01, required for in-cell assays
|
| |
| Components: |
Part # |
Component |
Size |
| |
4673-500-01 |
FITC-NAD |
250 µl |
| |
4674-250-01 |
Cell Permeabilization Reagent |
250 µl |
| |
| Physical State: |
Provided in solution at a concentration of 250 µm in deionized water. There is a 1:1 stoichiometry for incorporation; one FITC label for each NAD molecule. |
| |
| Extinction Coefficient: |
21,000 at 265nm |
| |
| Purity: |
95% by HPLC |
| |
| Storage: |
Store at -80°C in working aliquots to avoid repeated freeze-thawing. |
FITCNAD for use with enzymes requiring NAD as a substrate or cofactor. A number of proteins, including poly (ADP-ribose) polymerase (PARP), and the SIR2 family of NAD(+)-dependent histone/protein deacetylases, use NAD as a substrate for their function. FITC conjugated NAD permits the direct measurement of PARP and other NAD-dependent enzymes by fluorescence microscopy or by incorporation of fluorescent-labeled poly (ADP-ribose) onto histones attached to a 96 well plate.
