| Source: |
Purified from E. coli containing a recombinant plasmid harboring the hOGG1 gene. |
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| Unit Definition: |
One unit of hOGG1 catalyzes the cleavage of 1 pmole of a
32P-oligonucleotide probe in 1 hour at 37°C at an 8-oxoG/C
base pair within an oligonucleotide duplex.
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| Assay Conditions: |
4 pmoles of 32P-8-oxoG oligonucleotide
probe, 4 pmoles of complementary oligonucleotide, 1X REC™
Buffer 6 (20 mM Tris-Cl (pH 8.0), 1 mM DTT, 1 mM EDTA, 0.1 mg/ml
BSA) for 1 hour at 37°C in a reaction volume of 20 µl.
Cleavage products are resolved by 20% denaturing polyacrylamide
gel electrophoresis and detected by autoradiography.
|
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| Applications: |
Detection of oxidative DNA damage
hOGG1 FLARE™ Assay
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| Storage: |
Store in working aliquots at -20°C in a manual defrost freezer to avoid repeated freeze-thaws. The enzyme is supplied in 20 mM Tris-Cl (pH7.8), 1 mM EDTA, 100 mM NaCl, 1 mM DTT, 50% glycerol. |
