| Source: |
Purified from E. coli containing a recombinant plasmid harboring the human APE gene. |
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| Unit Definition: |
One unit is the amount of enzyme required to cleave an AP-site oligonucleotide within an oligonucleotide duplex at the rate of 1 pmol/hour at 37°C.
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| Assay Conditions: |
1X RECTM Buffer 7 (10 mM HEPES-KOH (pH 7.4), 100 mM KCl, 10 mM MgCl2), 4pmoles AP site oligonucleotide (Cat# 3851-100-01) labeled with 32P, 4 pmoles Oligo Complement B (Cat# 3849-100-02), and 1 unit human AP endonuclease in a 20 µl reaction volume and incubated for 1 hour at 37°C.
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| Applications: |
CometAssayTM/FLARETM
AP site cleavage
Redox activation of transcription factors
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| Storage: |
Freeze in working aliquots at -20 C or -80 C in a manual defrost freezer to avoid repeated freeze-thawing. |
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| Control: |
Freeze in working aliquots at -20°C in a manual defrost freezer to avoid repeated freeze-thawing. The enzyme is supplied in buffer containing 50% glycerol.
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