Shopping Cart » DIVAATM     » Cell Invasion     » PathClear® BME     » PARP     » CometAssayTM     » HT 8-oxo-dG ELISA  Home » DNA Damage DNA Damage Human DNA Polymerase ß We have good news to share with you regarding our Human DNA Polymerase ß. Our efforts to improve the expression and purification of the protein have succeeded, and we are now able to provide the product in greater amounts at significant cost savings. We have doubled the number of units in the smaller size, and we now offer larger sizes at dramatically reducted cost per unit activity. Human DNA Polymerase ß is constitutively expressed in cells and is thought to participate in the repair of DNA. The enzyme functions by filling in gaps in DNA, possibly formed following base excision repair. The activity of DNA Polymerase ß is not affected by aphidocolin. Unit Definition: One unit is the amount of enzyme required to catalyze the incorporation of 1 nmole of dNTP into an acid-insoluble form in 60 minutes at 37°C.   Assay Conditions: 1X REC™ Buffer 8 (50 mM Tris-Cl (pH 8.8) (3900-200-08), 10 mM MgCl2 , 100 mM KCl, 1.0 mM DTT, 1% glycerol), 50 µM dCTP, 50 µM dGTP, 50 µM dTTP, 50 µM α- 32P-dATP, and 10 µg of activated DNA in a reaction volume of 50 µl are incubated for 15 min at 37°C.   Applications: Gap filling DNA labeling Excision repair research     Human DNA Polymerase Kit Components: Part # Component Size   3900-200-08 10X REC Buffer 8 200 µl   4018-250 REC 5X Loading Buffer 250 µl   4019-1 REC water 1 ml   4020-050-02 Human DNA Beta-polymerase Contol DNA 10 µl   4020-050-04 Aphidicolin 10 µl   4020-100-01 Human DNA Beta-polymerase 100 units   Storage: Store at -20°C in a manual defrost freezer. The enzyme is supplied in buffer containing 50% glycerol. Top   Ordering Information Catalog #: Price: 4020-01K-EB Human Beta Polymerase Enzyme & Buffer, 1000 Units $895.00 4020-500-EB Human Beta Polymerase Enzyme & Buffer, 500 Units $570.00 4020-100-K Human Beta Polymerase Kit, 100 Units $290.00 4020-100-EB Human Beta Polymerase Enzyme & Buffer, 100 Units $225.00 Trevigen Products are now available for ordering through Download Protocol Select Protocol 4020-100-EB 4020-100-K Related Products Human AP Endonuclease (APE/Ref-1) Human Fen-1 Human DNA Ligase IV/XRCC4 Tetramer TREVIGEN INC. :: 8405 HELGERMAN CT. :: GAITHERSBURG, MD 20877 1.800.TREVIGEN :: 1.800.873.8443 :: (FAX) 1.301.560.4973 :: INFO@TREVIGEN.COM