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IIC: TACS® In Situ Apoptosis Detection Kits

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Is it recommended that Trevigen NF Mounting Media (cat# 4865-25) be used with TACS® In Situ Apoptosis Detection kits using either diaminobenzidine or TACS Blue Label®? Is it possible to use mounting media from other companies?

Why is TACS Blue Label™ fading?

Why does the blue label appear green?

Why does the blue label appear very weak?

Why is my Blue Stain being obscured by Nuclear Fast Red or Red Counter Stain C?

What is the difference between Cytonin™ and Proteinase K?

Which method or kit is recommended to detect apoptosis in frozen brain (hippocampus) samples from a heat stressed animal? Are there any pre-treatments for thick tissue sections? Is a free floating method required?

Do you have a procedure for in situ labeling of apoptotic cells in bone and cartilage?

Do you have a supplementary protocol for LR White Embedded Tissue (Electron Microscopy) for use with the TACS® 2 TdT Core Kit?

Do you have a reference or procedure for labeling bacteria for DNA fragmentation?

Which TACS® Kit is recommended for retinal tissues?

A paraffin embedded section was first stained using Vector Nova RED substrate kit (for peroxidase) to detect a cytoplase protein by immunohistochemistry. The section was then double labeled using TACS™ 2 TDT Kit. After completing the TACS™ protocol the red color stained by immunohistochemistry disappeared. What is the reason?

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