IIIA: CometAssay® Kit (cat# 4250-050-K)
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Will the CometAssay® (4250-050-K) work with solid tissue? If so how? Are there recommendations to reduce SYBR® Green I (4250-050-05) fading? Is it possible to combine the Anti-fade and SYBR® Green I (4250-050-05) Solutions? Do you recommend use of the CometAssay® (4250-050-K) with whole blood? How long can the CometSlide™ (4250-050-03) be stored before applying SYBR® Green I (4250-050-05) Staining Solution? What is the optimal filter set for SYBR® Green I (4250-050-05)? What image analysis tools are available for scoring comets? Why is there no staining in the positive control? What is the importance of pH for the electrophoresis buffer? Why are negative controls showing more DNA damage than expected in adherent cells? What is the effect of light on the CometAssay®? Will a ZEISS Axioplan/Axiovert microscope work for viewing a CometSlide™? How long can a CometSlide™ (4250-050-03) be stored? Is there a convenient step to stop the CometAssay® and resume the next day due to time constraints? Does it matter if the same pH is used for alkali unwinding and alkaline electrophoresis? Is it possible to differentiate between apoptotic and necrotic cells using the CometAssay®? Why are comet tails in positive control cells smaller than expected? Is it necessary to use a coverslip with a CometSlide™ (4250-050-03)?
The high throughput comet slide (CometSlide™ HT 4252-200-01) has 20 sample wells; is it possible to re-use the slide if not all wells were utilized? Are there protocols for removing the plant cell wall prior to performing the CometAssay®? The protocol for the CometAssay® Silver Staining Kit (4254-200-K) is designed for two well slides. Can the 100 µl volumes be decreased for twenty well slides (4252-200-01)? Do you have information or reference using CometAssay® for measuring oxidant stress in blood other than red blood cells? Will any commercial horizontal gel electrophoresis device work for electrophoresis? What alternatives to SYBR® Green are suitable for the CometAssay®? Why is there loss of agarose disks from the CometSlide™? << Return To FAQ Section List
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