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IIIC: PARP Assay

Click on the question below to view the detailed answer.


What is the protein concentration of the PARP enzyme? What is the specific activity? What is the purity of the enzyme?

Does the enzyme recognize a particular form of DNA damage?

What are the NAD (4667-50-04) and biotinylated NAD (4670-500-01) concentrations in the Universal Colorimetric PARP Assay kit?

How do the Universal Colorimetric PARP Assay Kit(4677-096-K) and Universal Chemiluminescent PARP Assay Kits (4676-096-K) work?

Do the PARP assay kits measure all possible PARP activity?

What are the absorbances for analysis in the Universal Colorimetric PARP Assay kit (4677-096-K), i.e. blue versus yellow precipitates?

Do the histones used in your PARP assay kit contain DNA? If that is not the case what is the activator of the PARP activity in this assay?

Are there substitutes for HCl in the Universal Colorimetric PARP Assay kit (4677-096-K)?

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