- Colorimetric readout
- 96 well format
- Sensitive – detects 10 mU PARP/well
- Assay Time ~3 hrs
Poly (ADP-ribose) polymerase (PARP) catalyzes the NAD+ dependent addition of poly(ADP-ribose) to itself and adjacent nuclear proteins, such as histones, in response to DNA damage. PARP contributes to the sequence of events that occurs during DNA base excision repair. Whereas PARP-mediated induction of necrosis can occur by extensive depletion of the intracellular NAD+ pool, the cleavage of PARP-1 promotes apoptosis by preventing DNA repair-induced survival and by blocking energy depletion-induced necrosis. Moreover, PARP inhibition promotes chemosensitization and radiosensitization of tumors. Trevigen's HT Universal PARP Assay Kits measure the incorporation of biotinylated poly(ADP-ribose) onto histone proteins in a 96-well strip well format. This assay is ideal for the determination of IC50 values of known or suspected PARP inhibitors. For your convenience, Trevigen provides histone-coated strip wells. This format significantly reduces assay time.
- Assay inhibitors and activators of PARP activity
- Determination of IC50 values for PARP Inhibitors
- Histone-Coated Strip Wells
- PARP Buffer and Cocktail
- Colorimetric Detection Reagents
- Activated DNA
- Strep HRP and Diluent
- 3-Aminobenzamide (known PARP inhibitor)
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