FEATURES:
- Colorimetric readout
- 96 well format
- Sensitive – detects 10 mU PARP/well
- Assay Time ~3 hrs
Poly (ADP-ribose) polymerase (PARP) catalyzes the NAD+ dependent addition of poly(ADP-ribose) to itself and adjacent nuclear proteins, such as histones, in response to DNA damage. PARP contributes to the sequence of events that occurs during DNA base excision repair. Whereas PARP-mediated induction of necrosis can occur by extensive depletion of the intracellular NAD+ pool, the cleavage of PARP-1 promotes apoptosis by preventing DNA repair-induced survival and by blocking energy depletion-induced necrosis. Moreover, PARP inhibition promotes chemosensitization and radiosensitization of tumors. Trevigen's HT Universal PARP Assay Kits measure the incorporation of biotinylated poly(ADP-ribose) onto histone proteins in a 96-well strip well format. This assay is ideal for the determination of IC50 values of known or suspected PARP inhibitors. For your convenience, Trevigen provides histone-coated strip wells. This format significantly reduces assay time.
APPLICATIONS:
- Assay inhibitors and activators of PARP activity
- Determination of IC50 values for PARP Inhibitors
KIT COMPONENTS:
- PARP-HSA
- Histone-Coated Strip Wells
- PARP Buffer and Cocktail
- Colorimetric Detection Reagents
- Activated DNA
- Strep HRP and Diluent
- 3-Aminobenzamide (known PARP inhibitor)
REFERENCES:
1. Satoh MS, Lindahl T. 1992. Role of poly(ADP-ribose) formation in DNA repair. Nature 356: 356–8.
2. Golstein P, Kroemer G. 2007. Cell death by necrosis: Towards a molecular definition. Trends Biochem Sci 32: 37-43.
3. D'Amours D, Sallmann FR, Dixit VM, Poirer GG. 2001. Gain-of-function of poly (ADP-ribose) polymerase-1 upon cleavage by apoptotic proteases: implications for apoptosis. J Cell Science 114: 3771-78.
4. Eliasson M, Sampei K, Mandir AJ. 1997. Poly(ADP-ribose) polymerase gene disruption renders mice resistant to cerebral ischemia. Nat Med 3:1089- 95.
5. Jagtap P, Szabó C. 2005. Poly(ADP-ribose) polymerase and the therapeutic effects of its inhibitors. Nat Rev Drug Discov. 4:421-40.
6. Pieper AA, Verma A, Zhang J, Snyder SH. 1999. Poly(ADP-ribose) polymerase, nitric oxide and cell death. Trends Pharmacol Sci 20:171-81.
7. Thiemermann C, Bowes J, Myint FP, Vane JR. 1997. Inhibition of the activity of poly(ADP-ribose) synthase reduces ischemia-reperfusion injury in the heart and skeletal muscle. Proc Natl Acad Sci USA 94:679-83.
8. Kauppinen TM, Swanson RA. 2005. Poly(ADP-ribose) polymerase-1 promotes microglial activation, proliferation, and matrix metalloproteinase-9-mediated neuron death. J Immunol. 174:2288-96.
9. http://www.trevigen.com/MarketingFlyers/JDS_PARP2008_Poster.pdf
Kit Components: | 
