Cultrex® Basement Membrane Extract
Cultrex® Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. The extract gels at 37°C to form a reconstituted basement membrane. The major components of BME include laminin, collagen IV, entactin, and heparin sulfate proteoglycan. Trevgen's BME typically ranges in protein concentrations from 14 to 16 mg/ml. BME is commonly used to maintain stem and precursor cells in an undifferentiated state during cell culture and BME hydrogels function to induce differentiation of epithelial, endothelial and smooth muscle cells in vitro. BME has also been employed in cell attachment, neurite outgrowth, angiogenesis, and in vitro cell invasion assays. BME is available with and without Phenol Red. Cultrex® BME is also available in PathClear® and HC20+™ varieties.
SHARED SPECIFICATIONS:
- Endotoxin (LPS) Concentration: ≤ 8 EU/ml
- Source: Murine Engelbreth-Holm-Swarm (EHS) tumor
- Storage Buffer: Dulbecco’s Modified Eagle’s medium containing 10 μg/ml gentamycin sulfate +/- phenol red.
- Storage/Stability: Product is stable for a minimum of 3 months from date of shipment when stored at –20 °C in a manual defrost freezer. For optimal stability, store at –80 °C in aliquots. Keep Frozen; repeated freeze-thaws will destroy product integrity
- Trevgen's BME typically ranges in protein concentrations from 14 to 16 mg/ml.
FUNCTIONAL ASSAYS :
- Tube Formation Assay: Basement Membrane Extract promotes formation of capillary-like structures by human (HUVEC, HMVEC) or mouse (SVEC4-10) endothelial cells.
- 3-D Culture: Basement Membrane Extract promotes differentiation of a human epithelial cell line derived from mammary gland (MCF-10A) and human prostate (PC-3) into acinar structures.
STANDARD STERILITY TESTING:
- No bacterial or fungal growth detected after incubation at 37 °C for 14 days following USP XXIV Chapter 71 sterility test.
- No mycoplasma contamination detected by PCR.
- Endotoxin concentrations ≤ 8 EU/ml by LAL assay.
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