3-D Culture Matrix™ reduced growth factor (RGF) BME
3-D Culture Matrix™ reduced growth factor (RGF) basement membrane extract (BME) provides the foundation for cells to grow in three dimensions, allowing for the formation of acinar and other hollow structures in vitro. Trevigen's 3-D Culture Matrix™ products enable investigators to employ an innovative in vitro approach to modeling cell responses in in vivo-like environments, which cannot be observed in standard 2-D cultures1-3.
Specifications:
CONCENTRATION:
12–18 mg/ml
SOURCE:
Murine Engelbreth-Holm-Swarm (EHS) tumor.
STORAGE BUFFER:
Dulbecco's Modified Eagle's medium containing 10 μg/ml gentamicin sulfate and no phenol red.
STORAGE/STABILITY:
Product is stable for a minimum of 3 months from date of shipment when stored at -20°C in a manual defrost freezer. For optimal stability, store at -80 °C in aliquots. Avoid repeated freeze/thaw cycles.
Material Qualification:
FUNCTIONAL ASSAY:
- Acinar structure formation: 3-D Culture Matrix™ BME promotes differentiation of a human epithelial cell line derived from mammary gland (MCF-10A) into acinar structures.
- Tube Formation Assay: BME promotes formation of capillary-like structures by human (HUVEC, HMVEC) or mouse (SVEC4-10) endothelial cells.
STERILITY TESTING :
- No bacterial or fungal growth detected after incubation at 37 °C for 14 days following USP XXIV Chapter 71 sterility test.
- No mycoplasma contamination detected by PCR.
- Endotoxin concentrations ≤ 8 EU/ml by LAL assay.
REFERENCES:
1. Benton, G., and J. George. 2008. Using 3-D culture for high-throughput analysis of cytotoxicity and proliferation. Am Biotechnol Lab 26:24-7.
2. Benton G., E. Crooke, and J. George. 2009. Laminin-1 induces E-cadherin expression in 3-dimensional cultured breast cancer cells by inhibiting DNA methyltransferase 1 and reversing promoter methylation status. FASEB J. 23:3884-95.
3. Benton G, J. George, H.K. Kleinman, I.P. Arnaoutova. 2009. Advancing science and technology via 3D culture on basement membrane matrix. J Cell Physiol. 221:18-25.
3-D Culture Matrix™ BME links:
http://cancerres.aacrjournals.org/content/68/15/6241.long
http://cancerres.aacrjournals.org/content/70/14/5706.long#methods | 
