Cultrex® Mouse Laminin I

Laminin I is an extracellular matrix protein which contains a number of functional domains that allow it to assemble into sheets. Trevigen’s mouse Laminin I is purified from the murine EHS sarcoma. This highly purified preparation increases cell adhesion, migration, proliferation, and differentiation.1 It is composed of α1β1γ1 chains with a total Mr of 800,000 and is used for coating culture dishes.

Embryonic stem (ES) cells express receptors for laminin. Thus, the use of laminin as a growth matrix should be considered when peptide growth factors present in more complex hydrogels might adversely impact experimental results. In pure form, laminin I is sufficient to support excellent ES cell growth in the undifferentiated state in the absence of feeder cells2.

Specifications:

CONCENTRATION:

1 mg/ml

SOURCE:

Murine Engelbreth-Holm-Swarm (EHS) tumor

STORAGE BUFFER:

Dulbecco’s Modified Eagle’s medium containing 10 μg/ml gentamycin sulfate.

STORAGE/STABILITY:

Store at -20°C or -80°C in a manual defrost freezer. Avoid repeated freeze/thaw cycles.

PURITY:

> 90% by SDS-PAGE gel

Material Qualification:

FUNCTIONAL ASSAY:

Supports the attachment (at least 50%) of MG63 human osteosarcoma cells to a
laminin-coated plate at a coating solution concentration of 20 μg/ml.

STERILITY TESTING:

• No bacterial or fungal growth detected after incubation at 37 °C for 14 days following USP XXIV Chapter 71 sterility test.
• No mycoplasma contamination detected by PCR
• Endotoxin concentrations ≤ 20 EU/ml by LAL assay.

REFERENCES:

1. Benton G., E. Crooke, and J. George. 2009. Laminin-1 induces E-cadherin expression in 3-dimensional cultured breast cancer cells by inhibiting DNA methyltransferase 1 and reversing promoter methylation status. FASEB J. 23:3884-95.
2. Xu, C., M.S Inokuma., J. Denham, K. Golds, P. Kundu, J.D. Gold, and M.K. Carpenter. 2001. Feeder-free growth of undifferentiated human embryonic stem cells. Nat. Biotechnol. 19:971–974.