TACS® Annexin V-FITC Kit

The TACS® Annexin V-FITC Kit allows rapid, specific, and quantitative identification of apoptosis in individual cells. Annexin V is a calcium-dependent phospholipid binding protein. During apoptosis, an early and ubiquitous event is the exposure of phosphatidylserine at the cell surface. The annexin V is supplied with an optimized Binding Buffer and propidium iodide. Propidium iodide may be used on unfixed samples to determine the population of cells that have lost membrane integrity, an indication of late apoptosis or necrosis.

FEATURES:

• Fast, sensitive detection of apoptotic cells.
• Includes propidium iodide for discriminating apoptotic and necrotic (or late apoptotic) cells.

APPLICATIONS :

• Flow cytometry.
• In situ detection of apoptotic cells.

RELATED PRODUCTS:

• TACS® Annexin V-Biotin Kit
• DePsipher™ Mitochondrial Potential Assay
• MitoShift™ Mitochondrial Potential Assay
• FlowTACS™ Kit
• TACS® 2 TdT FITC Kit

STORAGE:

Store at 4°C.

REFERENCES:

1. Kaetzel, M.A. and J.R. Dedman. 1995. Annexins: novel Ca2+ dependent regulators of membrane function. NIPS 10:171-174.
2. Koopman, G., C.P.M. Reutelinsperger, G.A.M. Kuijten, R.M.J. Keehnen, S.T. Pals, and M.H.J. van Oers. 1994. Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis. Blood 84(5):1415-1420.
3. Martin, S.J., C.P.M. Reutelinsperger, A.J. McGahon, J.A. Rader, R.C.A.A. van Schie, D.M. LaFace, and D.R. Green. 1995. Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: inhibition by overexpression of Bcl-2 and Abl. J. Exp. Med. 182:1545-1556.

RECENT CITATIONS:

1. Glutathione depletion is necessary for apoptosis in lymphoid cells independent of reactive oxygen species formation Rodrigo Franco, Mihalis I. Panayiotidis, and John A. Cidlowski J. Biol. Chem., Oct 2007; 282(42): 30452-65.
2. Selective Regulation of Bone Cell Apoptosis by Translational Isoforms of the Glucocorticoid Receptor Nick Z. Lu, Jennifer B. Collins, Sherry F. Grissom, and John A. Cidlowski Mol. Cell. Biol., Oct 2007; 27(20): 7143-60.
3. MAPK and heat shock protein 27 activation are associated with respiratory syncytial virus induction of human bronchial epithelial monolayer disruption Divyendu Singh, Kelly L. McCann, and Farhad Imani Am J Physiol Lung Cell Mol Physiol, Aug 2007; 293: L436 - L445.
4. Effects on neurite outgrowth and cell survival of a secreted fibroblast growth factor binding protein upregulated during spinal cord injury Elena Tassi, Sharon Walter, Achim Aigner, Rafael H. Cabal-Manzano, Ranjan Ray, Paul J. Reier, and Anton Wellstein Am J Physiol Regulatory Integrative Comp Physiol, Aug 2007; 293: R775 - R783.