TACS® 2 TdT In situ Apoptosis Detection Kits
FEATURES:
- TdT based in situ labeling.
- Cation optimization for high signal and low background.
- Complete kit provides either DAB, TACS Blue Label™, or fluorescence detection options.
- Includes exclusive Cytonin™ permeabilization reagent.
- Includes TACS-Nuclease™ control reagents.
APPLICATIONS:
- In situ detection of apoptosis (by TUNEL) in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.
- Light microscopy
- Fluorescence microscopy
- Flow cytometry
TACS® 2 TdT Kits utilize Trevigen’s unique cation optimization system to enhance labeling within particular tissues. The TACS® 2 TdT Kits also employ a proprietary labeling buffer that contains no toxic components (e.g. sodium cacodylate). A highly purified form of the TdT enzyme is included for the enzymatic incorporation of biotinylated nucleotides. Biotin labeling is detected using streptavidin-horseradish peroxidase, and colorimetric substrates
diaminobenzidine (DAB), or TACS Blue Label™. For fluorescent detection, a fluorescein conjugate of streptavidin is used and visualized by epifluorescence microscopy.
These complete kits provide all the reagents required for labeling including two permeabilization reagents, labeling and detection reagents, stop buffers, counterstain, and TACS-Nuclease™ reagents for generating positive controls with your own samples. Please refer to our components
listing for details.
The core kit is for researchers familiar with in situ labeling who are already set up for immunohistochemistry. A replenisher kit is also available for users of our TACS® kits who have used up the limiting reagents within their kit. The core kit provides a cost effective replacement of the reagents that are used most quickly.
ITEMS NOT INCLUDED:
Reagents for dehydration and rehydration of paraffin embedded sections, H2O2, methanol,
PBS buffer, mounting medium, and disposables.
STORAGE:
Components are stored at -20°C, 4°C, and room temperature.
Kit Components: |
