APPLICATIONS:
Suitable for Western Blot Analysis and immunocytochemistry. For Western blotting, a starting dilution of 1:1,000 is recommended, whereas for IC, a starting dilution of 1:100 is recommended.
Histone H2AX is a 14 kDa ubiquitous member of the H2A histone family that contains an evolutionarily conserved SQ motif at the C-terminus in eukaryotes. Serine 139 within this motif becomes rapidly phosphorylated to yield a form known as γ -H2AX in response todouble-strand DNA damage and apoptosis. Phosphorylation reaches half its maximum between 1-3 minutes after DNA damage occurs, and hundreds to several thousand molecules of γ-H2AX are present per double-strand break. This antibody is unique inonly detecting double-strand DNA breaks.
PHYSICAL STATE:
Rabbit serum containing polyclonal antibody raised against synthetic Phosphorylated peptide. Provided at 8 μg/μl in phosphate buffered saline with 0.01% sodium azide.
SPECIFICITY:
Recognizes mammalian, yeast, D. melanogaster, and X. laevis γ-H2AX.
STORAGE:
Freeze in working aliquots at -20°C to avoid repeated freeze-thawing.
REFERENCES:
1. Mahadevaiah, S.K., J.M. Turner, F. Baudat, E.P. Rogakou, P. de Boer, J. Blanco-Rodriguez, M. Jasin, S. Keeney, W.M. Bonner, P.S. Burgoyne. 2001. Recombinational DNA double-strand breaks in mice precede synapsis. Nature Gen 27:271-6.
2. Rogakou, E.P., W.Nieves-Neira, C.Boon, Y.Pommier, and W.M.Bonner. 2000. Initiation of DNA fragmentation during apoptosis induces phosphorylation of H2AX histone at serine139. J Biol Chem275:9390-5.
3. Rogakou, E.P., C. Boon, C. Redon, W.M. Bonner. 1999. Megabase chromatin domains involved in DNA double-strand breaks in vivo. J Cell Biol 146:905-16.
4. Rogakou, E.P., D.R. Pilch, A.H. Orr, V.S. Ivanova, W.M. Bonner. 1998. DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139. J Biol Chem 273:5858-68. |
