Suitable for Western Blot Analysis and immunocytochemistry. For Western blotting, a starting dilution of 1:1,000 is recommended, whereas for IC, a starting dilution of 1:100 is recommended.
Histone H2AX is a 14 kDa ubiquitous member of the H2A histone family that contains an evolutionarily conserved SQ motif at the C-terminus in eukaryotes. Serine 139 within this motif becomes rapidly phosphorylated to yield a form known as γ -H2AX in response todouble-strand DNA damage and apoptosis. Phosphorylation reaches half its maximum between 1-3 minutes after DNA damage occurs, and hundreds to several thousand molecules of γ-H2AX are present per double-strand break. This antibody is unique inonly detecting double-strand DNA breaks.
Rabbit serum containing polyclonal antibody raised against synthetic Phosphorylated peptide. Provided at 8 μg/μl in phosphate buffered saline with 0.01% sodium azide.
Recognizes mammalian, yeast, D. melanogaster, and X. laevis γ-H2AX.
Freeze in working aliquots at -20°C to avoid repeated freeze-thawing.
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