For Western and dot blotting, an antibody dilution of 1:1000 is recommended. For ELISA at 1:4000 antibody dilution is recommended. Empirical determination of antibody dilutions will be required for optimum results.
A rabbit polyclonal antibody raised against poly(ADP-ribose) (PAR) polymer. The anti-PARpolyclonal antibody can be used to detect ribosylated proteins by immunodetection.Trevigen's PARP treated control protein (4500-10-P ) and PAR polymer (catalog # 4336-100-01 ) may be used as positive controls.
This polyclonal antibody is a purified IgG fraction in 1X PBS containing 50% glycerol.
This polyclonal antibody detects free PAR and poly-ribosylated proteins.
-20°C (manual defrost freezer)
1. Affar, E.B., et al. 1998. Immunodot blot method for the detection of poly (ADP-ribose) synthesized in vitro and in vivo. Anal. Biochem. 259:280-283.
2. Shah, G.M., et al. 1995. Methods for biochemical study of poly (ADP-ribose) metabolism in vitro and in vivo. Anal. Biochem. 227:1-13.
3. Kinders JK, Hollingshead M, Khin S, Rubinstein L, Tomaszewski JE, Doroshow JH, Parchment RE, and the National Cancer Institute Phase 0 Clinical Trials Team. 2008. Preclinical Modeling of a Phase 0 Clinical Trial: Qualification of a Pharmacodynamic Assay of Poly (ADP-Ribose) Polymerase in Tumor Biopsies of Mouse Xenografts. Clin Cancer Res 14 :6877-85