Anti-phosphorylated Histone H2AX (g-H2AX) Polyclonal Antibody

Background:

Histone H2AX is a 14 kDa ubiquitous member of the H2A histone family that contains an evolutionarily conserved SQ motif at the C-terminus in eukaryotes. Serine 139 within this motif becomes rapidly phosphorylated to yield a form known as g -H2AX in response to double-strand DNA damage and apoptosis. Phosphorylation reaches half its maximum between 1-3 minutes after DNA damage occurs, and hundreds to several thousand molecules of g-H2AX are present per double-strand break. This antibody is unique in only detecting double-strand DNA breaks.

Physical State:

Peptide affinity purified rabbit polyclonal antibody raised against synthetic phosphorylated peptide (CKATQA[pS]QEY). Provided at 0.5 µg/µl in phosphate buffered saline with 50% glycerol.

Specificity:

Recognizes human and mouse g-H2AX.

Storage:

-20°C.

Applications:

Suitable for Western blotting and immunocytochemistry. For Western blot analysis, a starting dilution of 1:1000 is recommended, whereas for IC, a starting dilution of 1:100 is recommended. Empirical determination of antibody concentration is required for optimal results.