Anti-BPDE Monoclonal Antibody (clone 8E11)
|Catalog #||Product Name||Size||Price|
|4360-MC-100||Anti-BPDE Monoclonal Antibody (clone 8E11)||100 µg||$340.00|
Benzo[a]pyrene diol epoxide
The antibody recognizes both free polycyclic aromatic hydrocarbons (PAHs) and DNA adducts. In an inhibition assay, the binding of the antibody to BPDE adducts was inhibited by BPDE-I-DNA, BPDE-I-dG, BPDE-I tetraol but not by BPDE-II-DNA1.
The antibody was raised against BPDE-I-G coupled to BSA.
This antibody is provided from mouse ascites, provided at 1 mg/ml in PBS, 50% glycerol.
Stored at -20°C. For long term storage, freeze in working aliquots at -20°C in a manual defrost freezer to avoid repeated freeze-thawings.
Competitve ELISA and immunoprecipitation. The antibody may be used to investigate exposure to environmental and occupational pollutants. PAHs are released into the environment following incomplete combustion of organic materials. Human exposure to PAHs comes from various occupational, environmental, dietary and medicinal sources. Exposure to this group of compounds is believed to be carcinogenic.
1. Santella, R.M., C.D. Lin, W. L. Cleveland, and I.B. Weinstein. 1984. Monoclonal antibodies to DNA modified by a benzo[a]pyrene diol epoxide. Carcinogenesis 5:373-377.