Benzo[a]pyrene diol epoxide
The antibody recognizes both free polycyclic aromatic hydrocarbons (PAHs) and DNA adducts. In an inhibition assay, the binding of the antibody to BPDE adducts was inhibited by BPDE-I-DNA, BPDE-I-dG, BPDE-I tetraol but not by BPDE-II-DNA1.
The antibody was raised against BPDE-I-G coupled to BSA.
This antibody is provided from mouse ascites, provided at 1 mg/ml in PBS, 50% glycerol.
Stored at -20°C. For long term storage, freeze in working aliquots at -20°C in a manual defrost freezer to avoid repeated freeze-thawings.
Competitve ELISA and immunoprecipitation. The antibody may be used to investigate exposure to environmental and occupational pollutants. PAHs are released into the environment following incomplete combustion of organic materials. Human exposure to PAHs comes from various occupational, environmental, dietary and medicinal sources. Exposure to this group of compounds is believed to be carcinogenic.
1. Santella, R.M., C.D. Lin, W. L. Cleveland, and I.B. Weinstein. 1984. Monoclonal antibodies to DNA modified by a benzo[a]pyrene diol epoxide. Carcinogenesis 5:373-377.
2. Lee, B.M. and R. Santella. 1988. Quantitation of protein adducts as a marker of genotoxic exposure: immunologic detection of benzo[a]pyrene-globin adducts in mice. Carcinogenesis 9:1773-1777.
3. Strickland, P.T., D. Kang, E.D. Bowman, A. Fitzwilliam, T.E. Downing, N. Rothman, J.D. Groopman, and A. Weston. 1994. Identification of 1-hydroxypyrene glucuronide as a major pyrene metabolite in human urine by synchronous fluorescence spectroscopy and gas chromatography-mass spectrometry. Carcinogenesis 15:483-487.
4. Kang, D.H., N. Rothman, M.C. Poirier, A. Greenberg, C.H. Hsu, B.S. Schwartz, M.E. Baser, J.D. Groopman, A. Weston, and P.T. Strickland. 1995. Interindividual differences in the concentration of 1-hydroxypyrene-glucuronide in urine and polycyclic aromatic hydrocarbon- DNA adducts in peripheral white blood cells after charbroiled beef consumption. Carcinogenesis 16:1079-1085.
5. Kang , D.H., N. Rothman, S.-H. Cho, H.S. Lim, H.-J. Kwon, S.-M. Kim, B. Schwartz, and P.T. Strickland. 1995. Association of exposure to polycyclic aromatic hydrocarbons (estimated from job category) with concentration of 1-hydroxypyrene glucuronide in urine from workers at a steel plant. Occupational and Environmental Medicine 52:593-599.