DNA Damage / H2AX

gamma H2AX Pharmacodynamic Assay



Catalog #Product NameSizePrice
4418-096-K gamma H2AX Pharmacodynamic Assay96 tests$1250.00

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Trevigen's gamma H2AX Pharmacodymanic assay is the first commercially available kit for the study of double strand DNA breaks through the detection of gamma H2AX- a phosphorylated histone historically proven as a highly specific and sensitive molecular marker for double strand DNA damage detection. The assay documents differences in gamma H2AX levels in peripheral blood mononuclear cells, cultured cells, and tissue biopsies and is available as a complete reagent kit with chemiluminescent detection. 

“Trevigen’s gamma H2AX pharmacodynamic assay is a very useful assay providing precise quantitation of gamma H2AX formation. The assay is easy to perform, fast, and displays good linear range and excellent reproducibility between replicate samples.” Says Alexei Degretev from Tufts University, one of the initial beta-testers of the product, testing compounds on the HeLa cell line.  

FEATURES:

  • Chemiluminescent, non-radioactive ELISA
  • Pre-coated 96 well capture antibody plate
  • Dynamic range from 10 pM to 800pM
  • Sensitivity with 5 pM of gamma H2AX

APPLICATIONS:

  • quantitates gamma H2AX levels in PBMC, cultured cells and tissue biopsies

KIT COMPONENTS:

  • 96-stripwell pre-coated plate with 3 film sealers
  • gamma H2AX Standard
  • Assay Buffer
  • H2AX IgM Detecting Antibody
  • Goat anti-Mouse IgM HRP Conjugate
  • Cell Lysis Reagent
  • Jurkat Cell Lysate Control
  • 25X Wash Buffer
  • PeroxyGlow™ A
  • PeroxyGlow™ B