Cultrex® Stem Cell Qualified Human Vitronectin, PathClear®
Description:
Vitronectin provides a functionally defined and effective feeder-free surface for the attachment and maintenance of embryonic stem cells in a pluripotent state. It is an extracellular, soluble, disulfide-linked dimer, composed of a 75 kDa and a 65 kDa
peptide chain with a total molecular weight of 140 kDa. Vitronectin is a major plasma glycoprotein that promotes cellular adhesion and spreading, inhibits the membrane damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. Vitronectin, along with collagen IV, fibronectin, and laminin can support robust, long term proliferation of human embryonic stem cells in the undifferentiated state. Vitronectin can be used for coating tissue culture surfaces to promote cell adhesion, proliferation and differentiation, or as an additive for serum-free media.
Specifications:
Concentration:
~1 mg/ml
Source:
Human plasma
Storage Buffer:
10 mM Sodium Phosphate, pH 7.7, 8 M Urea, 5 mM EDTA, 0.5 M NaCl.
Storage/Stability:
Product is stable for a minimum of 3 months from date of shipment when stored at –80 °C.
Repeated freeze-thaws will destroy product integrity.
Materials Qualification:
Functional Assay:
- Promotes the attachment of H9 human embryonic stem cells.
- Effectively maintains human embryonic stem cells in a pluripotent state as evidenced by intracellular staining for the stem cell markers Oct-4 and Nanog.
*Sterility Testing:
- No bacterial or fungal growth detected after incubation at 37°C for 14 days following USP XXIV Chapter 71 sterility testing protocols.
- No mycoplasma contamination detected by PCR.
- Stem Cell Qualified Human Vitronectin, PathClear® is tested negative by PCR for different human pathogenic viruses including EBV, HAdV, Hantaan, HCMV, Hepatitis A, Hepatitis B, Hepatitis C, HHV 6, HHV 8, HIV1, HIV2, HSV 1, HSV 2, HTLV 1, HTLV 2, LCMV, Seoul, Sin Nombre, VZV.
- Endotoxin concentration < 20 EU/ml by LAL assay.
Safety Statement: Cultrex® Human Vitronectin, PathClear® is purified from human source
material and therefore should be treated as potentially infectious and handled at Biological Safety
Level 2 to minimize exposure. |
