TACS® Annexin V-Biotin Kit
FEATURES:
- Fast, sensitive detection of apoptotic cells.
- Includes propidium iodide for discriminating apoptotic and necrotic (or late apoptotic) cells.
- Provides flexibility in fluorophore choice.
APPLICATIONS :
- Flow cytometry
- In situ detection of apoptotic cells
- Can be fixed after binding to live cells
The TACS® Annexin V-Biotin Kit allows rapid, specific, and quantitative identification of apoptosis in individual cells. Annexin V is a calcium-dependent phospholipid binding protein. During apoptosis, an early and ubiquitous event is the exposure of phosphatidylserine at the cell surface. The annexin V is supplied with an optimized Binding Buffer and propidium iodide. Propidium iodide may be used on unfixed samples to determine the population of cells that have lost membrane integrity, an
indication of late apoptosis or necrosis. Both early and late apoptotic cells in tissues, which are either cultured or freshly isolated, can be detected using TACS® Annexin V-Biotin.
RELATED PRODUCTS:
- TACS® Annexin V-FITC Kit
- DePsipher™ Mitochondrial Potential Assay
- MitoShift™ Mitochondrial Potential Assay
- FlowTACS™ Kit
- TACS® 2 TdT FITC Kit
STORAGE:
Store at 4°C.
REFERENCES:
1. Kaetzel, M.A. and J.R. Dedman. 1995. Annexins: novel Ca2+ dependent regulators of membrane function. NIPS 10:171-174.
2. Koopman, G., C.P.M. Reutelinsperger, G.A.M. Kuijten, R.M.J. Keehnen, S.T. Pals, and M.H.J. van Oers. 1994. Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis. Blood 84(5):1415-1420.
3. Martin, S.J., C.P.M. Reutelinsperger, A.J. McGahon, J.A. Rader, R.C.A.A. van Schie, D.M. LaFace, and D.R. Green. 1995. Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: inhibition by overexpression of Bcl-2 and Abl. J. Exp. Med. 182:1545-1556.
4. J Baleriola, J., T. Suárez, and E.J. de la Rosa. 2010. DNA-PK promotes the survival of young neurons in the embryonic mouse retina. Cell Death Differ. 2010 May 7. [Epub ahead of print].
Kit Components: |
