Cultrex® Stem Cell Qualified Laminin I

Cultrex® Stem Cell Qualified Laminin I provides a functionally defined and effective feeder-free surface for the attachment and maintenance of embryonic stem cells in a pluripotent state , thereby enabling its use for growth promotion or study of stem cell differentiation. This highly purified preparation of mouse Laminin I is composed of α1β1γ1 chains with a total Mr of 800,000.

Specifications:

CONCENTRATION:

1 mg/ml

Source:

Murine Engelbreth-Holm-Swarm (EHS) tumor.

Storage Buffer:

Dulbecco’s Modified Eagle’s Medium with 10 μg/ml gentamicin sulfate.

Storage:

Store at -20°C or -80°C in a manual defrost freezer.

Purity:

Purity >90% by SDS-PAGE.

Concentration:

1 mg/ml

Sterility Testing:

• No bacterial or fungal growth detected after incubation at 37°C for 14 days following USP XXIV, Chapter 71 sterility testing.
• No mycoplasma contamination detected by PCR
• Endotoxin concentrations < 20 EU/ml by LAL assay.

Material Qualification:

• Promotes the attachment of H9 human embryonic stem cells.
• Effectively maintains human embryonic stem cells in a pluripotent state as evidenced by intracellular staining for the stem cell markers Oct-4 and Nanog.

Coating procedure for Stem Cell Propagation:
The recommended working concentration is 10 μg/cm2 of growth surface depending on cell
type.