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HT 8-oxo-dG ELISA kit II

8-Oxo-2'-deoxyguanosine (8-oxo-dG), a frequently used biomarker of oxidative DNA damage, is removed from DNA by the base excision repair pathway, and subsequently transported into saliva, urine and plasma. In order to measure the total pool of 8-oxo-dG, Trevigen has developed a validated competitive ELISA kit that quantifies 8-oxo-dG in DNA, plasma, urine and saliva.  Trevigen’s HT 8-oxo-dG ELISA kit II, employs a 96 strip well pre coated with 8-oxo-dG, an anti-8-oxo-dG monoclonal mouse antibody, an HRP conjugated secondary antibody, and colorimetric detection substrate to construct a high throughput assay flexible for your experimental design. The 8-OHdG monoclonal antibody binds competitively to 8-oxo-dG immobilized on pre-coated wells and in solution. Antibody bound to 8-oxo-dG in the sample is washed away while antibody bound to 8-oxo-dG attached to the well is retained. Detection of the retained antibody is performed using a HRP conjugate and colorimetric substrate. Product formation is inversely proportional to the amount of 8-oxo-dG present in the sample.

FEATURES:

• Colormetric, non-radioactive format
• High throughput 96 strip wells
• Dynamic range from 3.13 nM to 200 nM (0.89 ng/ml to 56.7 ng/ml)
• Sensitivity at 2 nM (0.57 ng/ml) 8-OHdG

APPLICATIONS:

For the detection and quantitation of 8-hydroxy-2’-deoxyguanosine (8-OHdG) in DNA, plasma, urine and saliva samples.

KIT COMPONENTS:

• 8-OHdG Standard, 20 µm
  
• Assay Diluent
• 8-OHdG monoclonal antibody
• Goat anti-Mouse IgG-HRP
• Pre-coated 96-stripwell plate 
• 100X Cation
• DNase I (5 Units/µl)
• Alkaline Phosphatase (1 Unit/µl)
• TACS-Sapphire

REFERENCES:

1. Nunomura, A., Perry, G., Pappolla, M.A., Wade, R., Hirai, K., Chiba, S., and Smith, M.A. (1999) Journal of Neuroscience 19:1959-1964.
2. Sancar, A., Lindsey-Boltz, L. A., Unsal-Kacmaz, K., and Linn, S. (2004) Annu. Rev. Biochem. 73: 39-85.
3. Wood, M. L., Dizdaroglu, M., Gajewski, E. and Essigmann, J. M. (1990) Biochemistry, 29, 7024-7032.
4. Marnett, L.J. (2000) Carcinogenesis. 21 (3): 361-370.
5. Tsou, T., Chen, C., Liu, T., and Yang, J. (1996) Carcinogenesis 17, 103-108.