Glutathione Reductase Assay Kit
FEATURES:
- Suitable for cell lysates, erythrocyte lysates, and tissue homogenates
- Each sample takes only 6 minutes
- Sufficient reagents for 100 assays and 10 standard curves
APPLICATIONS :
Determination of glutathione reductase activity.
Trevigen’s Glutathione Reductase Assay Kit is a higher volume assay designed to measure cellular levels of Glutathione Reductase, an enzyme which catalyzes the reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH) (figure1). GSH is an important intracellular antioxidant that reacts with oxygen free radicals and organic peroxides and acts as a substrate for other detoxification enzymes such as glutathione peroxidase and glutathione S-transferase. The activity of glutathione reductase is an important measure of the antioxidant status of the cell. Glutathione reductase requires NADPH for its activity, resulting in the reduction of GSSG to GSH and the corresponding oxidation of NADPH to NADP+.
Trevigen’s Glutathione Reductase Assay takes less than 6 minutes to perform and is based on the measurement of the loss in absorbance at 340 nm due to the conversion of NADPH to NADP+. The kit contains everything that you need to perform the assay on 100 samples and its corresponding blanks, each in duplicate, as well as 10 standard curves with each point performed in duplicate. In total, each kit can generate data on over 500 data points. The NADPH is provided lyophilized in 10 vials, each containing sufficient NADPH for generating one standard curve and for determining glutathione reductase levels in 10 experimental samples.
REFERENCES:
1. Nordman, T., L. Xia, L. Bjorkhem-Bergman, A. Damdimopoulos, I. Nalvarte, E.S. Arner, G. Spyrou, L.C. Eriksson, M. Bjornstedt, and J.M. Olsson. 2003. Regeneration of the antioxidant ubiquinol by lipoamide dehydrogenase, thioredoxin reductase and glutathione reductase. Biofactors 18:45-50.
2. Droge, W. 2003. Oxidative stress and aging. Adv. Exp. Med. Biol. 543:191-200.
3. Higuchi, Y. 2003. Chromosomal DNA fragmentation in apoptosis and necrosis induced by oxidative stress. Biochem. Pharmacol. 15:1527-1535. |
