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Molecular Biology

OligoTAGTM Kinase Kit

The OligoTAG kit provides all the necessary reagents (except labeled nucleotide) to generate a high specific activity 5’ end labeled DNA sequence. The provided T4 polynucleotide kinase attaches ATP to the 5’ hydroxyl group of oligonucleotides, single-stranded DNA, or RNA. Removal of 5’ phosphate may be required for some DNA substrates to allow labeling. The kit can also be used to add non-radiolabeled nucleotides to the 5’ end of DNA.

Applications:
  • Labeling oligonucleotides
  • Adding 5’-phosphate to oligonucleotides for ligation
  •  
    Components: Part # Component Size
      4019-1 REC water 1 ml
      9500-25-01 10X Buffer 100 µl
      9500-25-05 T4 Polynucleotide 25 µl
     
    Storage: Store all reagents at -20°C in a manual defrost freezer. Avoid repeated freeze-thawing.
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    Ordering Information
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    Catalog #: Price:

    9500-25-K
    OligoTAG Kinase Kit,
    25 Reactions
    $150.00
    Add to Cart

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