Shopping Cart » DIVAATM     » Cell Invasion     » PathClear® BME     » PARP     » CometAssayTM     » HT 8-oxo-dG ELISA  Home » Oxidative Damage Oxidative Damge HT Superoxide Dismutase Assay Kit Superoxide Dismutase (SOD) catalyzes the dismutation of the superoxide radical (O2-) into hydrogen peroxide (H202) and elemental oxygen (O2), and as such, provides an important defense against the toxicity of superoxide radical. Over expression of SOD protects murine fibrosarcoma cells from apoptosis and promotes cell differentiation (1). SOD also inhibits adriamycin-induced apoptosis in murine peritoneal macrophages (2). Superoxide ions, generated from the conversion of xanthine to uric acid and hydrogen peroxide by xanthine oxidase, converts NBT to NBT-diformazan. NBT-diformazan absorbs light at 550 nm. SOD reduces the superoxide ion concentration and thereby lowers the rate of NBT-diformazan formation. The extent of reduction in the appearance of NBT-diformazan is a measure of SOD activity present in your experimental sample. The assay is free of interference by other catalytic activities, and is ideal for assaying SOD in mammalian cell lysates. Unlike some other assay kits for SOD, this system is not greatly disturbed by trace metals, and after a simple calculation, the percent inhibition of the formation of NBT-diformazan by SOD is converted to the relative activity of the sample. Features: 96 well format for high throughput screening Suitable for mammalian cells Suitable for the assay of (Mn2+)-SOD, (Fe2+)-SOD, and (Cu/Zn)-SOD   Applications: Calculating SOD activity by measuring the reduction of NBT-diformazan in cell extracts.   Components: Part # Component Size   7501-500-01 SOD Standard, 50 units/ ™l 50 µl   7501-500-02 10X SOD Buffer 20 ml   7501-500-03 Xanthine Oxidase (XOD) 3 ml   7501-500-04 10X Xanthine Solution 2 ml   7501-500-05 20% Triton X-100 1 ml   7501-500-06 WST-1 Reagent 3 ml   7512-100-06 96-well plates 5 plates   Storage: Store components at -20°C, 4°C and room temperature.   References: 1. Zhao, Y., K.K. Kiningham, S.M. Lin, and D.K. St. Clair. 2001. Overexpression of MnSOD protects murine fibrosarcoma cells from apoptosis and promotes a differentiation program upon treatment with 5- azacytidine. Antioxid. Redox Signal. 3:375-386. 2. Dominguez-Rodriguez, J.R., et.al. 2001. In vivo inhibition by antioxidants of adriamycin-induced apoptosis in murine peritoneal macrophages. Anticancer Res. 21:1869-1872. Top   Ordering Information Catalog #: Price: 7501-500-K HT SOD Assay Kit, 500 tests $495.00 Trevigen Products are now available for ordering through Download Protocol Related Products HT Glutathione Reductase Assay Kit Superoxide Dismutase Assay Kit Glutathione Reductase Assay Kit HT Glutathione Assay Kit TREVIGEN INC. :: 8405 HELGERMAN CT. :: GAITHERSBURG, MD 20877 1.800.TREVIGEN :: 1.800.873.8443 :: (FAX) 1.301.560.4973 :: INFO@TREVIGEN.COM