Rat Mesenchymal Stem Cells

Mesenchymal Stem Cells (MSC), also known as marrow stromal cells, are a self-renewing population of multipotent cells present in bone marrow and many other adult tissues. MSC can be isolated from bone marrow by adherence to plastic, and can differentiate into multiple lineage- specific cells that form bone, fat, cartilage, muscle, neuronal cells and tendon. Due to their multilineage potential, they can be useful tools for a wide range of therapeutic and basic research, including transplantation studies and studies examining the repair of cardiac tissue, bone, cartilage, and tendons often using 3-D matrices. The generation of dependable information that can be shared between laboratories requires a consistent, highly purified source of MSC that have maintained their multipotency, as demonstrated by their ability to differentiate to different tissue types. Isolation of MSC is both a time and resource consuming process which can now be eliminated. A readily available and qualified source of cells will substantially decrease MSC preparation time required prior to experimentation.

Trevigen's RMSC are provided as a frozen ampoule containing 1 x 10⁶ passage 3 cells that are greater than 99% pure. These cells can be maintained in an undifferentiated state when grown in Trevigen's qualified RMSC Medium supplemented with RMSC qualified FBS or induced to differentiate into adipogenic or osteogenic phenotype when growth media are supplemented with reagents contained in Trevigen's differentiation kits. Trevigen's RMSC can undergo 10 doublings without alteration in cell morphology or differentiation potential.

Rat Mesenchymal Stem Cells Starter Kit

Trevigen's stem cell starter kit comes with the stem cells described about as well as RMSC medium, and RMSC Fetal Bovine Serum. 

Rat Mesenchymal Stem Cells Replenisher Kit

The replenisher kit comes with the RMSC medium and RMSC Fetal Bovine Serum to support continued research.  

Cultrex® Mesenchymal Stem Cell Osteogenic Differentiation Kit

Mesenchymal Stem Cells, a self-renewing population of multipotent cells, can differentiate into osteogenic cells that will deposit and mineralize extracellular matrix proteins, which is required for the formation of new bone. Measuring the amount of mineralized matrix is an established method for evaluating osteogenic differentiation. Trevigen’s Mesenchymal Stem Cell Osteogenic Differentiation Kit contains reagents optimized to direct mesenchymal stem cells grown on Cultrex® Rat Collagen I4 to undergo osteogenic differentiation in a defined growth medium supplemented with ascorbic acid, β-glycerol phosphate, dexamethasone within 14 days. Osteogenic differentiation is detected by staining with Alizarin Red S5. Alizarin red will stain the calcium deposits that occur when differentiated mesenchymal stem cells mineralize the extracellular matrix. The differentiation of mesenchymal stem cells into osteogenic cells can be a useful tool for understanding mechanisms involved in bone formation. It is also used for identifying potential treatments for osteoporosis, bone repair and genetic disorders such as osteogenesis imperfecta.

Cultrex® Mesenchymal Stem Cell Adipogenic Differentiation Kit

Adipogenesis is the process by which fat is formed. Fat formation plays a role in obesity, cardiovascular disease, and metabolic disorders. It also has a role in the maintenance of the bone marrow cavity. Adipocytes are derived from mesenchymal stem cells (MSC), which are a self-renewing population of multipotent cells present in bone marrow and many other adult tissues that can differentiate into multiple lineage-specific cells that form bone, fat, cartilage, muscle and tendon. Differentiation of primary cells, specifically MSC, into adipocytes can be a useful tool for understanding the mechanisms involved in adipogenesis leading eventually to interventions designed to prevent obesity.

Trevigen’s adipogenic differentiation kit follows traditional methods of adipogenic differentiation, by growing MSC in medium supplemented with insulin, isobutyl methyl xanthine (IBMX), indomethacin and dexamethasone. The kit will induce adipogenic differentiation of MSC within 14-17 days. Adipogenic differentiation is detected by staining with Oil Red O. Oil Red O will stain lipid containing vacuoles in mesenchymal stem cells that have undergone adipogenic differentiation.