Cultrex® Stem Cell Qualified Laminin I

Cultrex® Stem Cell Qualified Laminin I provides a functionally defined and effective feeder-free surface for the attachment and maintenance of embryonic stem cells in a pluripotent state , thereby enabling its use for growth promotion or study of stem cell differentiation. This highly purified preparation of mouse Laminin I is composed of α1β1γ1 chains with a total Mr of 800,000.

Cultrex® Stem Cell Qualified Protein Set

Trevigen’s Cultrex® Stem Cell Qualified Protein Set was created to help researchers in choosing optimal coating conditions for feeder-free culture of stem cells of interest.
Cultrex® Stem Cell Qualified Protein Set contains 5 different extracellular matrix proteins:

• Human Basement Membrane Extract (BME)
• Human Fibronectin
• Human Vitronectin
• Mouse Reduced Growth Factor BME
• Mouse Laminin I

Each of these proteins was qualified in feeder-free cell culture to support human embryonic stem cells in the undifferentiated state for multiple passages.

Cultrex® Stem Cell Qualified Human Fibronectin, PathClear®

Fibronectin provides a functionally defined and effective feeder-free surface for the attachment and maintenance of embryonic stem cells in a pluripotent state. It is an extracellular matrix protein that is found abundantly in blood, connective tissues, and remodeled matrices also associated with the epithelial to mesenchymal transition of migratory cells--including tumor cells with stem cell-like properties.  Fibronectin performs essential functions in collagen fibrillogenesis, and as either a general cell adhesion molecule or as a modulator in binding between cell surfaces and the extracellular matrix. Fibronectin matrix assembly is essential for normal vertebrate development, and apparently contributes to the generation of tumor metastases by supporting the establishment and persistence of premetastaticniches. Fibronectin is secreted as a disulfide-linked dimer of 230-270 kDa, comprised of three types of repeating modules that mediate interactions with extracellular matrix components (including fibronectin itself), and cells via integrins and other fibronectin receptors.

Cultrex® Stem Cell Qualified Human Vitronectin, PathClear®

Vitronectin provides a functionally defined and effective feeder-free surface for the attachment and maintenance of embryonic stem cells in a pluripotent state. It is an extracellular, soluble, disulfide-linked dimer, composed of a 75 kDa and a 65 kDa
peptide chain with a total molecular weight of 140 kDa. Vitronectin is a major plasma glycoprotein that promotes cellular adhesion and spreading, inhibits the membrane damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. Vitronectin, along with collagen IV, fibronectin, and laminin can support robust, long term proliferation of human embryonic stem cells in the undifferentiated state. Vitronectin can be used for coating tissue culture surfaces to promote cell adhesion, proliferation and differentiation, or as an additive for serum-free media.