8-Oxo-2'-deoxyguanosine (8-oxo-dG), a frequently used biomarker of oxidative DNA damage, is removed from DNA by the base excision repair pathway, and subsequently transported into saliva, urine and plasma. In order to measure the total pool of 8-oxo-dG, Trevigen has developed a validated competitive ELISA kit that quantifies 8-oxo-dG in DNA, plasma, urine and saliva in the ELISA Kit II.  Trevigen’s HT 8-oxo-dG ELISA kit II, employs a 96 strip wells pre coated with 8-oxo-dG, an anti-8-oxo-dG monoclonal mouse antibody, an HRP conjugated secondary antibody, and colorimetric detection substrate to construct a high throughput assay flexible for your experimental design. The 8-OHdG monoclonal antibody binds competitively to 8-oxo-dG immobilized on pre-coated wells and in solution. Antibody bound to 8-oxo-dG in the sample is washed away while antibody bound to 8-oxo-dG attached to the well is retained. Detection of the retained antibody is performed using an HRP conjugate and colorimetric substrate. Product formation is inversely proportional to the amount of 8-oxo-dG present in the sample.

Researchers looking to quantify 8-oxo-dG in DNA will prefer the second generation kit to the first, as the first generation does not quantify 8-oxo-dG in DNA. Both kits come in colormetric format and offer a robust assay flexible to individual research.